Recombinant Human MAPK1 Protein, His-tagged MAPK1-2417H

Recombinant Human MAPK1 Protein, His-tagged

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Recombinant Human MAPK1 Protein, His-tagged

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Cat.No. : MAPK1-2417H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human MAPK1 Protein (Tyr25-Ser360) with a N-His tag was expressed in E. coli.
Description : This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Source : E. coli
Species : Human
Tag : N-His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 42.8 kDa
Accurate Molecular Mass: 43 kDa
Protein length : Tyr25-Ser360
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : > 95%
Applications : Cell culture; Activity Assays.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE :

WB :

Bioactivity :

Binding activity

Gene Name : MAPK1 mitogen-activated protein kinase 1 [ Homo sapiens (human) ]
Official Symbol : MAPK1
Synonyms : MAPK1; mitogen-activated protein kinase 1; ERK; p38; p40; p41; ERK2; ERT1; NS13; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; mitogen-activated protein kinase 1
MAP kinase 1
MAP kinase 2
MAPK 2
extracellular signal-regulated kinase 2
mitogen-activated protein kinase 2
protein tyrosine kinase ERK2
EC 2.7.11.24
Gene ID : 5594
mRNA Refseq : NM_002745
Protein Refseq : NP_002736
MIM : 176948
UniProt ID : P28482
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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