Recombinant Human MAPK3

• Cat.No.: MAPK3-28699TH
• Product Overview: Recombinant full length protein corresponding to amino acids 2-379 of Human ERK1, with an N-terminal proprietary tag; predicted MWt 69.8 kDa inclusive of tag.Residue A232 of the fusion protein is equivalent to A2 of the native enzyme. Protease cleavage si

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
• Protein length: 378 amino acids
• Molecular Weight: 69.800kDa inclusive of tags
• Source: E. coli
• Biological activity: Specific activity: 379.25 units/ml;621.73 Units/mg
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 7.50Constituents:0.79% Tris HCl, 9.24% Sucrose, 0.88% Sodium chloride, 0.003% EGTA, 0.1% Beta mercaptoethanol, 0.002% Brij, 0.012% Benzamidine, 0.003% PMSF
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDK WRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHN MLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKV DFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGG GGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGE GAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQ ILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLY KLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWY RAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHY LDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKV AWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYL EQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP
• Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: MAPK3 mitogen-activated protein kinase 3 [ Homo sapiens ]
• Official Symbol: MAPK3
• Synonyms: MAPK3; mitogen-activated protein kinase 3; PRKM3; ERK1; p44erk1; p44mapk
• Gene ID: 5595
• mRNA Refseq: NM_001040056
• Protein Refseq: NP_001035145
• MIM: 601795
• Uniprot ID: P27361
• Chromosome Location: 16p11.2
• Pathway: ALK1 signaling events, organism-specific biosystem; ARMS-mediated activation, organism-specific biosystem; ATF-2 transcription factor network, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Activation of the AP-1 family of transcription factors, organism-specific biosystem
• Function: ATP binding; MAP kinase activity; MAP kinase activity; nucleotide binding; phosphatase binding