| Cat.No. : |
PCSK9-352H |
| Product Overview : |
Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # AAI66619). Predicted N-terminus: Gln 31. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 72.0 kDa. The protein migrates as 20 kDa and 66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His & Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human LDL R, His Tag at 10 μg/mL (100 μL/well) can bind Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated with a linear range of 2-31 ng/mL (QC tested).
Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated can bind anti-Human PCSK9 Mab, Human IgG1 with a linear range of 0.05-1.5 ng/mL. The biotinylated PCSK9 is coated at 1 ug/mL (100 uL/well) on a streptavidin coated plates (Routinely tested).
Immobilized Human VLDL R, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated with a linear range of 10-156 ng/mL (Routinely tested).
FACS analysis shows that Biotinylated Human PCSK9, His Tag (Cat. No. PC9-H82E7) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 1.502-2.378 μg/mL. |
| Molecular Mass : |
72.0 kDa |
| Protein length : |
Gln 31 - Gln 692 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>92% as determined by reduced SDS-PAGE. |
| Notes : |
Please avoid repeated freeze-thaw cycles. |
| Stability : |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Figure : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.