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Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated
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Cat.No. : | PCSK9-352H |
Product Overview : | Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # AAI66619). Predicted N-terminus: Gln 31. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 72.0 kDa. The protein migrates as 20 kDa and 66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Source : | HEK293 |
Species : | Human |
Tag : | His & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human LDL R, His Tag at 10 μg/mL (100 μL/well) can bind Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated with a linear range of 2-31 ng/mL (QC tested). Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated can bind anti-Human PCSK9 Mab, Human IgG1 with a linear range of 0.05-1.5 ng/mL. The biotinylated PCSK9 is coated at 1 ug/mL (100 uL/well) on a streptavidin coated plates (Routinely tested). Immobilized Human VLDL R, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human PCSK9 Protein, His & Avi-tagged, Biotinylated with a linear range of 10-156 ng/mL (Routinely tested). FACS analysis shows that Biotinylated Human PCSK9, His Tag (Cat. No. PC9-H82E7) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 1.502-2.378 μg/mL. |
Molecular Mass : | 72.0 kDa |
Protein length : | Gln 31 - Gln 692 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >92% as determined by reduced SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Figure : |
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Gene Name : | PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens ] |
Official Symbol : | PCSK9 |
Synonyms : | PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; NARC 1; NARC1; NARC-1; HCHOLA3; |
Gene ID : | 353175 |
MIM : | 607786 |
UniProt ID : | Q8NBP7 |
◆ Recombinant Protein
Pcsk9-209M | Recombinant Mouse Pcsk9 Protein, His-Avi-tagged, Biotinylated | +Inquiry |
PCSK9-86H | Recombinant Human PCSK9 Protein, His-tagged | +Inquiry |
PCSK9-035H | Recombinant Human PCSK9 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
Pcsk9-020M | Recombinant Mouse Pcsk9 Protein, MYC/DDK-tagged | +Inquiry |
PCSK9-03H | Recombinant Human PCSK9 Protein, His-tagged | +Inquiry |
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◆ Lysates
PCSK9-2306RCL | Recombinant Rat PCSK9 cell lysate | +Inquiry |
PCSK9-2564MCL | Recombinant Mouse PCSK9 cell lysate | +Inquiry |
PCSK9-2775RCL | Recombinant Rhesus PCSK9 cell lysate | +Inquiry |
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◆ Assay kits
Kit-1807 | PCSK9[Biotinylated]-LDLR Binding Assay Kit | +Inquiry |
Kit-1808 | PCSK9-LDLR TR-FRET Assay Kit | +Inquiry |
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