Recombinant Human PDGFA Protein

• Cat.No.: PDGFA-047H
• Product Overview: Recombinant human Platelet-derived growth factor AA ( rhPDGF-AA) Ser 87 – Thr211(Accession # P04085) was expressed in human 293 cells (HEK293).

Specification

• Description: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
• Source: HEK293 cells
• Species: Human
• Molecular Mass: rhPDGF-AA is a homodimeric, glycosilated, polypeptide chain containing 2×110 amino acids, and has a calculated MW of 29 kDa. In DTT-reduced SDS-PAGE, rhPDGF-AA protein migrates as 16.5 kDa poly peptide due to glycosylation.
• Endotoxin: Less than 0.01 ng endotoxin/ 1 µg rhPDGF-AA.
• Purity: >95% purity as determined by SDS-PAGE of reduced rhPDGF-AA.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhPDGF-AB should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: PDGFA platelet-derived growth factor alpha polypeptide [ Homo sapiens ]
• Official Symbol: PDGFA
• Synonyms: PDGFA; platelet-derived growth factor alpha polypeptide; platelet-derived growth factor subunit A; PDGF A chain; PDGF A; PDGF1; platelet derived growth factor alpha chain; PDGF-1; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; PDGF-A
• Gene ID: 5154
• mRNA Refseq: NM_002607
• Protein Refseq: NP_002598
• MIM: 173430
• UniProt ID: P04085