Recombinant Human PKM2 Protein, His-tagged

• Cat.No.: PKM2-78H
• Product Overview: Recombinant Human PKM2 Protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in 20 mM Tris-HCl (pH 8.0).
• Molecular Mass: ~60.2 kDa
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMSKPH SEAGTAFIQTQQLHAAMADTFLEHM CRLDIDSPPITARNTGIICTIGPAS RSVETLKEMIKSGMNVARLNFSHGT HEYHAETIKNVRTATESFASDPILY RPVAVALDTKGPEIRTGLIKGSGTA EVELKKGATLKITLDNAYMEKCDEN ILWLDYKNICKVVEVGSKIYVDDGL ISLQVKQKGADFLVTEVENGGSLGS KKGVNLPGAAVDLPAVSEKDIQDLK FGVEQDVDMVFASFIRKASDVHEVR KVLGEKGKNIKIISKIENHEGVRRF DEILEASDGIMVARGDLGIEIPAEK VFLAQKMMIGRCNRAGKPVICATQM LESMIKKPRPTRAEGSDVANAVLDG ADCIMLSGETAKGDYPLEAVRMQHL IAREAEAAMFHRKLFEELVRASSHS TDLMEAMAMGSVEASYKCLAAALIV LTESGRSAHQVARYRPRAPIIAVTR NPQTARQAHLYRGIFPVLCKDPVQE AWAEDVDLRVNFAMNVGKARGFFKK GDVVIVLTGWRPGSGFTNTMRVVPV P
• Endotoxin: <0.1 EU per ug of the protein as determined by the LAL method.
• Purity: >95%
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.1 mg/ml
• Official Full Name: Pyruvate kinase M1/2

Gene Information

• Official Symbol: PKM
• Synonyms: PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Gene ID: 5315
• mRNA Refseq: NM_001206796.
• Protein Refseq: NP_001193725
• MIM: 179050
• UniProt ID: P14618