Active Recombinant Human PKM2 Protein, N-His tagged

• Cat.No.: PKM2-34H
• Product Overview: Recombinant Human PKM2 Protein with N-His tag was expressed in E. coli.

Specification

• Description: Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, the last reaction of glycolysis. The M2 isozyme of pyruvate kinase, or PKM2 is the only isozyme expressed in cancer cells, and have been used clinically as tumor maker for detection of early cancer and monitor progress of cancer treatment.
• Source: E. coli
• Species: Human
• Tag: N-His
• Bio-activity: PKM2 activity was measured by an enzyme coupled assay utilizing lactic-acid dehydrogenase (LDH). PKM2 activity is reflected in the conversion of nicotinamide adenine dinucleotide acid (NADH) to nicotinamide adenine dinucleotide (NAD+). The amount of NADH is measured by colorimetric absorbance at 340 nm. The concentration of NADH can be calculated according to its extinction coefficient (6220/ M-1cm-1 at 340 nm). The purified PKM2 was activated by fructose 1,6-bisphosphate (FBP). The presence of FBP lowered the Km for substrate PEP by 20 fold.
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMSKPH SEAGTVRTATESFASDPILYRPVAV ALDTKGPEIRGSLGSKKGVNLPGAA VDLPAVSEKDIQDLKKMMIGRCNRA GKPVICATQMLESMIKKPRPFKCCS GAIIVLTKSGRSAHQVARYRPRAPI PAFIQTQQLHAAMADTFLEHMCRLD IDSPPITARNTGIICTIGPASRSVE TLKEMIKSGMNVARLNFSHGTHEYH AETIKNTGLIKGSGTAEVELKKGAT LKITLDNAYMEKCDENILWLDYKNI CKVVEVGSKIYVDDGLISLQVKQKG ADFLVTEVENGFGVEQDVDMVFASF IRKASDVHEVRKVLGEKGKNIKIIS KIENHEGVRRFDEILEASDGIMVAR GDLGIEIPAEKVFLAQTRAEGSDVA NAVLDGADCIMLSGETAKGDYPLEA VRMQHLIAREAEAAIYHLQLFEELR RLAPITSDPTEATAVGAVEASIAVT RNPQTARQAHLYRGIFPVLCKDPVQ EAWAEDVDLRVNFAMNVGKARGFFK KGDVVIVLTGWRPGSGFTNTMRVVP V
• Purity: > 98% by SDS-PAGE
• Usage: Useful for studying enzyme kinetics, screening for PKM2 modulators including activators and inhibitors, and selectivity profiling. Protein binding assay, antigen, ELSA, Western blot, crystallization, co-crystallization and crystal structure determination study.
• Storage: Upon delivery aliquot and store at -80 centigrade. Avoid multiple freeze-thaw cycles. The protein is stable for 12 months at -80 centigrade, for 2-4 weeks at 4 centigrade.
• Concentration: 0.5 mg/mL
• Storage Buffer: PKM2 with 2 mM DTT, 20% glycerol, 0.02% NaN3, 150 mM NaCl, 0.1 mM EDTA, 50 mM Tris-HCl pH7.5.

Gene Information

• Gene Name: PKM2 pyruvate kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: PKM2
• Synonyms: PKM2; pyruvate kinase, muscle; pyruvate kinase isozymes M1/M2; OIP3; PK3; THBP1; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic; PKM; TCB; CTHBP; MGC3932
• Gene ID: 5315
• mRNA Refseq: NM_001206797
• Protein Refseq: NP_001193726
• MIM: 179050
• UniProt ID: P14618

Reviews

12/19/2021

Accurate mass spectrometry, pivotal for precise proteomics analysis.

11/30/2019

Expert protein engineering support, indispensable for our project success.

09/28/2019

Precise protein quantification, ensures data accuracy in experiments.

Q&As

How do alterations in PKM2 expression or activity affect metabolic disorders such as diabetes?

Changes in PKM2 activity can impact glucose metabolism, relevant to metabolic disorders like diabetes.

How does PKM2 contribute to the regulation of apoptosis and cell survival?

PKM2 regulates cell survival and apoptosis, influencing cellular fate decisions.

How does PKM2 influence the Warburg effect in cancer cells?

PKM2 contributes to the Warburg effect, enabling cancer cells to preferentially utilize glycolysis for energy.

What role does PKM2 play in cancer cell metabolism and tumor growth?

PKM2 plays a crucial role in cancer metabolism, supporting rapid cell growth and proliferation.

How does PKM2 (Pyruvate Kinase M2) regulate glycolysis and energy production in cells?

PKM2 controls the rate of glycolysis, impacting energy production and metabolic flux in cells.

What is the impact of PKM2 on cellular responses to hypoxic conditions?

PKM2 helps cells adapt to hypoxia, altering metabolism to cope with low oxygen levels.

What are the mechanisms by which PKM2 modulates the immune response in the tumor microenvironment?

PKM2 modulates the immune response within the tumor microenvironment, affecting tumor immunity.