Recombinant Human PLA2G2A Protein (Asn21-Cys144), His tagged

• Cat.No.: PLA2G2A-4923H
• Product Overview: Recombinant human PLA2G2A (Asn21-Cys144) fused with His tag was expressed in E. coli.

Specification

• Description: Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 15.96 kDa
• Protein Length: Asn21-Cys144
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5 or lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: PLA2G2A phospholipase A2, group IIA (platelets, synovial fluid) [ Homo sapiens (human) ]
• Official Symbol: PLA2G2A
• Synonyms: PLA2G2A; phospholipase A2, group IIA (platelets, synovial fluid); PLA2B, PLA2L; phospholipase A2, membrane associated; NPS-PLA2; GIIC sPLA2; group IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; non-pancreatic secretory phospholipase A2; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Gene ID: 5320
• mRNA Refseq: NM_000300
• Protein Refseq: NP_000291
• MIM: 172411
• UniProt ID: P14555