Recombinant Human SART3 Protein (600-900 aa), His-tagged

• Cat.No.: SART3-1664H
• Product Overview: Recombinant Human SART3 Protein (600-900 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Cancer. Protein Description: Partial.

Specification

• Description: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity).By similarity4 Publications Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 35.8 kDa
• Protein length: 600-900 aa
• AA Sequence: QRKRARAEKKALKKKKKIRGPEKRG ADEDDEKEWGDDEEEQPSKRRRVEN SIPAAGETQNVEVAAGPAGKCAAVD VEPPSKQKEKAASLKRDMPKVLHDS SKDSITVFVSNLPYSMQEPDTKLRP LFEACGEVVQIRPIFSNRGDFRGYC YVEFKEEKSALQALEMDRKSVEGRP MFVSPCVDKSKNPDFKVFRYSTSLE KHKLFISGLPFSCTKEELEEICKAH GTVKDLRLVTNRAGKPKGLAYVEYE NESQASQAVMKMDGMTIKENIIKVA ISNPPQRKVPEKPETRKAPGGPMLL P
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: SART3 squamous cell carcinoma antigen recognized by T cells 3 [ Homo sapiens ]
• Official Symbol: SART3
• Synonyms: SART3; KIAA0156; RP11 13G14; SART-3; hSART-3; P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14; MGC138188
• Gene ID: 9733
• mRNA Refseq: NM_014706
• Protein Refseq: NP_055521
• MIM: 611684
• UniProt ID: Q15020