Recombinant Mouse Sart3 Protein, Myc/DDK-tagged

• Cat.No.: Sart3-5693M
• Product Overview: Purified recombinant protein of mouse full-length squamous cell carcinoma antigen recognized by T cells 3 (Sart3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation. Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites. May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly. May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair. May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 110.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sart3 squamous cell carcinoma antigen recognized by T cells 3 [ Mus musculus (house mouse) ]
• Official Symbol: Sart3
• Synonyms: SART3; squamous cell carcinoma antigen recognized; squamous cell carcinoma antigen recognized by T-cells 3; SART-3; mSART-3; tumor-rejection antigen SART3; AU045857; mKIAA0156
• Gene ID: 53890
• mRNA Refseq: NM_016926
• Protein Refseq: NP_058622
• UniProt ID: Q9JLI8