| Description : |
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
60.3 kDa |
| AA Sequence : |
MDNMSITNTPTSNDACLSIVHSLMC HRQGGESETFAKRAIESLVKKLKEK KDELDSLITAITTNGAHPSKCVTIQ RTLDGRLQVAGRKGFPHVIYARLWR WPDLHKNELKHVKYCQYAFDLKCDS VCVNPYHYERVVSPGIDLSGLTLQS NAPSSMMVKDEYVHDFEGQPSLSTE GHSIQTIQHPPSNRASTETYSTPAL LAPSESNATSTANFPNIPVASTSQP ASILGGSHSEGLLQIASGPQPGQQQ NGFTGQPATYHHNSTTTWTGSRTAP YTPNLPHHQNGHLQHHPPMPPHPGH YWPVHNELAFQPPISNHPAPEYWCS IAYFEMDVQVGETFKVPSSCPIVTV DGYVDPSGGDRFCLGQLSNVHRTEA IERARLHIGKGVQLECKGEGDVWVR CLSDHAVFVQSYYLDREAGRAPGDA VHKIYPSAYIKVFDLRQCHRQMQQQ AATAQAAAAAQAAAVAGNIPGPGSV GGIAPAISLSAAAGIGVDDLRRLCI LRMSFVKGWGPDYPRQSIKETPCWI EIHLHRALQLLDEVLHTMPIADPQP LDTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
