Recombinant Human SMAD4 293 Cell Lysate

• Cat.No.: SMAD4-1676HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for SMAD family member 4 (SMAD4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SMAD4 SMAD family member 4 [ Homo sapiens ]
• Official Symbol: SMAD4
• Synonyms: SMAD4; SMAD family member 4; MAD, mothers against decapentaplegic homolog 4 (Drosophila) , MADH4, SMAD, mothers against DPP homolog 4 (Drosophila); mothers against decapentaplegic homolog 4; DPC4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4; JIP; MADH4; MYHRS
• Gene ID: 4089
• mRNA Refseq: NM_005359
• Protein Refseq: NP_005350
• MIM: 600993
• UniProt ID: Q13485
• Chromosome Location: 18q21.1
• Pathway: ALK1 signaling events, organism-specific biosystem; ALK2 signaling events, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMP Signalling Pathway, organism-specific biosystem; BMP receptor signaling, organism-specific biosystem
• Function: contributes_to DNA binding; DNA binding; I-SMAD binding; R-SMAD binding; SMAD binding; chromatin binding; collagen binding; core promoter proximal region sequence-specific DNA binding; identical protein binding; protein binding; contributes_to protein binding transcription factor activity; protein homodimerization activity; contributes_to sequence-specific DNA binding; contributes_to sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity