Recombinant Human SRC, GST-tagged
Cat.No. : | SRC-191H |
- Specification
- Gene Information
- Related Products
Cat. No. : | SRC-191H |
Description : | Src (p60c-src) is a 60 kDa cytoplasmic tyrosine kinase with functions in the regulation of cell division, cell-matrix adhesion, and cell-cellcell adhesion. Src-GST family tyrosine kinases participate in signal transduction of different classes of membrane receptors, including receptor tyrosine kinases and G-protein coupeled receptors. The SrcGST has been generated by fusing GST N-terminal to human c-Src allowing expression inE. coliand purification by affinity chromatography with GSHbeads. The preparation is >90% homogenous in SDS-PAGE and has a specific activity of >3000 U/mg (1 U is defined as transfer of 1 pMol phosphate to the substrate peptide AAEEEIYGEFEAKKKK per minute at 30°C). The fusion protein has been successfully used to label phosphatase substrates. The GST-Tag facilitates the protein's application in typical GST pull-down assays. |
Source : | E. coli. |
Form : | Liquid. Supplied in 50 mM Tris-HCl pH 8.0, 100 mM NaCl and 1 mM DTT. |
Purity : | ≥90% by SDS-PAGE. |
Application : | > 650 U/µg (1 U is defined as transfer of 1 pMol phosphate to the substrate peptide AAEEEIYG-EFEAKKKK per minute at 30°C). |
Usage : | For in vitro use only. |
Storage : | Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles. |
Gene Name : | SRC v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) [ Homo sapiens ] |
Synonyms : | SRC; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian); ASV; SRC1; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase SRC; OTTHUMP00000174476; OTTHUMP00000174477; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; EC 2.7.10.2; pp60c-src; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogenehomolog |
Gene ID : | 6714 |
mRNA Refseq : | NM_005417 |
Protein Refseq : | NP_005408 |
MIM : | 190090 |
UniProt ID : | P12931 |
Chromosome Location : | 20q12-q13 |
Pathway : | Adherens junction; Endocytosis; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; Focal adhesion; Gap junction; GnRH signaling pathway; Tight junction; VEGF signaling pathway |
Function : | ATP binding; SH2 domain binding; SH3/SH2 adaptor activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; transferase activity |
Products Types
◆ Native Protein | ||
SRC-29697TH | Native Human SRC | +Inquiry |
◆ Lysates | ||
SRC-487HCL | Recombinant Human SRC cell lysate | +Inquiry |
SRC-585MCL | Recombinant Mouse SRC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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