Recombinant Human STAT3 Protein, His-tagged

• Cat.No.: STAT3-001H
• Product Overview: Recombinant full-length human STAT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Source: Insect cells
• Species: Human
• Tag: His
• Molecular Mass: 95 kDa
• Purity: >85%
• Applications: Kinase Assay, Western Blot
• Stability: 1 year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25% glycerol.

Gene Information

• Gene Name: STAT3
• Official Symbol: STAT3 signal transducer and activator of transcription 3 [ Homo sapiens (human) ]
• Synonyms: STAT3; signal transducer and activator of transcription 3; APRF; FLJ20882; MGC16063; APRF; HIES; ADMIO; ADMIO1; signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• Gene ID: 6774
• mRNA Refseq: NM_139276
• Protein Refseq: NP_644805
• MIM: 102582
• UniProt ID: P40763

Reviews

11/17/2022

Protein worked well in assay.

04/21/2021

Fast shipping, great customer service.

02/06/2018

Excellent purity, highly recommend.

Q&As

Are there any known inhibitors targeting STAT3 for therapeutic purposes?

Yes, there are several inhibitors targeting STAT3, including Stattic, S3I-201, and OPB-31121, which are being explored for their therapeutic potential in cancer and other diseases.

Can STAT3 activation influence cell proliferation and survival?

Yes, activated STAT3 can promote cell proliferation and survival by inducing the expression of genes like cyclin D1 and Bcl-xL.

How does STAT3 contribute to oncogenesis and tumor progression?

STAT3 can contribute to oncogenesis by promoting cell proliferation, survival, angiogenesis, and immune evasion, and by suppressing apoptosis in various cancer types.

How can the activation of STAT3 be detected in cells?

Activation of STAT3 can be detected using Western blotting with phospho-specific antibodies that recognize the tyrosine-phosphorylated form of STAT3.

How does STAT3 interact with other signaling pathways in the cell?

STAT3 can interact with other signaling pathways such as the MAPK, PI3K/AKT, and NF-κB pathways, leading to integrated cellular responses.

What are the primary upstream activators of STAT3?

The primary upstream activators of STAT3 are cytokines and growth factors, especially those that signal through the Janus kinase (JAK) family, such as IL-6 and EGF.

What role does STAT3 play in immune cell differentiation and function?

STAT3 plays a crucial role in the differentiation of Th17 cells, a subset of CD4+ T cells, and is also involved in the function of myeloid-derived suppressor cells (MDSCs) and regulatory T cells, influencing immune responses.