Recombinant Human STAT3 293 Cell Lysate
Cat.No. : | STAT3-1417HCL |
- Specification
- Gene Information
- Related Products
Description : | Antigen standard for signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) [ Homo sapiens ] |
Official Symbol : | STAT3 |
Synonyms : | STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3; APRF; DNA-binding protein APRF; acute-phase response factor; HIES; FLJ20882; MGC16063; |
Gene ID : | 6774 |
mRNA Refseq : | NM_213662 |
Protein Refseq : | ?NP_998827 |
MIM : | 102582 |
UniProt ID : | P40763 |
Chromosome Location : | 17q21 |
Pathway : | Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; |
Products Types
◆ Recombinant Protein | ||
STAT3-4148H | Recombinant Human STAT3 Protein, His (Fc)-Avi-tagged | +Inquiry |
Stat3-6170M | Recombinant Mouse Stat3 Protein, Myc/DDK-tagged | +Inquiry |
Stat3-2107R | Recombinant Rat Stat3 Protein, His-tagged | +Inquiry |
STAT3-2837H | Recombinant Human STAT3 Protein, His-tagged | +Inquiry |
STAT3-138H | Recombinant Human STAT3 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
STAT3-1418HCL | Recombinant Human STAT3 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (7)
Ask a questionYes, there are several inhibitors targeting STAT3, including Stattic, S3I-201, and OPB-31121, which are being explored for their therapeutic potential in cancer and other diseases.
Yes, activated STAT3 can promote cell proliferation and survival by inducing the expression of genes like cyclin D1 and Bcl-xL.
STAT3 can contribute to oncogenesis by promoting cell proliferation, survival, angiogenesis, and immune evasion, and by suppressing apoptosis in various cancer types.
Activation of STAT3 can be detected using Western blotting with phospho-specific antibodies that recognize the tyrosine-phosphorylated form of STAT3.
STAT3 can interact with other signaling pathways such as the MAPK, PI3K/AKT, and NF-κB pathways, leading to integrated cellular responses.
The primary upstream activators of STAT3 are cytokines and growth factors, especially those that signal through the Janus kinase (JAK) family, such as IL-6 and EGF.
STAT3 plays a crucial role in the differentiation of Th17 cells, a subset of CD4+ T cells, and is also involved in the function of myeloid-derived suppressor cells (MDSCs) and regulatory T cells, influencing immune responses.
Customer Reviews (3)
Write a reviewProtein worked well in assay.
Fast shipping, great customer service.
Excellent purity, highly recommend.
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