Recombinant Human Syntrophin, Alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component), His-tagged

• Cat.No.: SNTA1-4831H
• Product Overview: Recombinant human SNTA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SNTA1 is a member of the syntrophin gene family, which is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. Dystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing10% glycerol, 1mM DTT, 0.15M NaCl.
• Molecular Weight: 56.3 kDa (528aa), confirmed by MALDI-TOF.
• Purity: >80% by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSMASGRRA PRTGLLELRA GAGSGAGGER WQRVLLSLAE DVLTVSPADG DPGPEPGAPR EQEPAQLNGA AEPGAGPPQL PEALLLQRRR VTVRKADAGG LGISIKGGRE NKMPILISKI FKGLAADQTE ALFVGDAILS VNGEDLSSAT HDEAVQVLKK TGKEVVLEVK YMKDVSPYFK NSTGGTSVGW DSPPASPLQR QPSSPGPTPR NFSEAKHMSL KMAYVSKRCT PNDPEPRYLE ICSADGQDTL FLRAKDEASA RSWATAIQAQ VNTLTPRVKD ELQALLAATS TAGSQDIKQI GWLTEQLPSG GTAPTLALLT EKELLLYLSL PETREALSRP ARTAPLIATR LVHSGPSKGS VPYDAELSFA LRTGTRHGVD THLFSVESPQ ELAAWTRQLV DGCHRAAEGV QEVSTACTWN GRPCSLSVHI DKGFTLWAAE PGAARAVLLR QPFEKLQMSS DDGASLLFLD FGGAEGEIQL DLHSCPKTIV FIIHSFLSAK VTRLGLLA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Signaling mediated by p38-gamma and p38-delta
• Publications:
  p38γ MAPK contributes to left ventricular remodeling after pathologic stress and disinhibits calpain through phosphorylation of calpastatin (2019)

Gene Information

• Gene Name: SNTA1 syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component) [ Homo sapiens ]
• Official Symbol: SNTA1
• Synonyms: SNTA1; syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component); SNT1; LQT12; TACIP1; dJ1187J4.5; syntrophin, alpha 1 (dystrophin-associated protein A1, 59kD, acidic component); acidic alpha 1 syntrophin; alpha-1-syntrophin; Pro-TGF-alpha cytoplasmic domain-interacting protein 1; 59 kDadystrophin-associated protein A1 acidic component 1; syntrophin-1
• Gene ID: 6640
• mRNA Refseq: NM_003098
• Protein Refseq: NP_003089
• MIM: 601017
• UniProt ID: Q13424
• Chromosome Location: 14q11.2
• Function: PDZ domain binding; actin binding; calmodulin binding; protein binding

Citation

p38γ MAPK contributes to left ventricular remodeling after pathologic stress and disinhibits calpain through phosphorylation of calpastatin

Journal: The FASEB Journal    Data: 2019/12/1

Authors: Aminah A. Loonat, E. Denise Martin, James E. Clark

Article Snippet:Fractions that containedGST-calpastatinwerepooled anddialyzedovernight at 4°C against 232Lof buffer that contained 50mMTris, pH8.0, 50mM NaCl, 2 mMDTT.Fractions that containedGST-calpastatinwerepooled anddialyzedovernight at 4°C against 232Lof buffer that contained 50mMTris, pH8.0, 50mM NaCl, 2 mMDTT.. In vitro kinase assays Phosphorylation of substrates was performed in vitro in kinase buffer (25 mM Tris, pH 7.5, 5 mM b-glycerophosphate, 2 mM DTT, 0.2mMNa3VO4, and 10mMMgCl2) and contained 200 ng of active p38g, 1 mM ATP, ATP-g-S or 6-PhEt-ATPgS (BioLog, Minden, Germany), and 1 mg of recombinant ATF2 (Cell Signaling Technology), a1-syntrophin (Creative BioMart, Shirley, NY, USA), LDB3, or calpastatin substrate.. Reactions were incubated at 30°C for 30 min.Reactions were incubated at 30°C for 30 min.