| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors. |
| Form : |
20 mM Tris, pH 7.6 at 4 centigrade, 100 mM NaCl, 10% glycerol |
| Molecular Mass : |
8.9 kDa |
| Purity : |
> 95% by HPLC |
| Usage : |
Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can used in conjunction with wild type ubiquitin at combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of N-terminal biotin-Ub: wild type ubiquitin. |
| Storage : |
Store at -80 centigrade; avoid multiple freeze-thaw cycles. |
