Recombinant Human UB Protein, Biotin-tagged
Cat.No. : | UB-391H |
Product Overview : | Recombinant Human UB Protein is produced by Bacteria expression system. This protein is fused with a Biotin tag at the N-terminal. |
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Description : | A single biotin moiety is conjugated on the N-terminus of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of N-terminal biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput FRET assays to monitor substrate ubiquitination in assays screening E3 ubiquitin ligase inhibitors. |
Source : | Bacteria |
Species : | Human |
Tag : | Biotin |
Form : | 20 mM Tris, pH 7.6 at 4 centigrade, 100 mM NaCl, 10% glycerol |
Molecular Mass : | 8.9 kDa |
Purity : | > 95% by HPLC |
Usage : | Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can used in conjunction with wild type ubiquitin at combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of N-terminal biotin-Ub: wild type ubiquitin. |
Storage : | Store at -80 centigrade; avoid multiple freeze-thaw cycles. |
Synonyms : | Ubiquitin |
Synonyms : | Ubiquitin |
Products Types
◆ Recombinant Protein | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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