Recombinant Human USP12 293 Cell Lysate

Cat.No. : USP12-473HCL
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Species : Human
Source : HEK293
Tag : Non
Description : Antigen standard for ubiquitin specific peptidase 12 (USP12) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name USP12 ubiquitin specific peptidase 12 [ Homo sapiens ]
Official Symbol USP12
Synonyms USP12; ubiquitin specific peptidase 12; ubiquitin specific protease 12 , ubiquitin specific protease 12 like 1 , USP12L1; ubiquitin carboxyl-terminal hydrolase 12; ubiquitin thioesterase 12; deubiquitinating enzyme 12; ubiquitin thiolesterase 12; ubiquitin-hydrolyzing enzyme 1; ubiquitin specific protease 12 like 1; ubiquitin-specific-processing protease 12; UBH1; USP12L1;
Gene ID 219333
mRNA Refseq NM_182488
Protein Refseq NP_872294
UniProt ID O75317
Chromosome Location 13q12.13

Not For Human Consumption!

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