Recombinant Human VTN Protein, His-tagged

• Cat.No.: VTN-062H
• Product Overview: Recombinant human Vitronectin / VTN (rhVitronectin) Asp20-Leu478 (Accession # NP_000629.3 ) fused with a N-terminal 6×His tag was produced in human HEK 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-478 a.a.
• Description: The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
• Molecular Mass: rhVitronectin is fused with a 6×his tag at N-terminal and the protein has a calculated MW of 53 kDa. DTT- reduced protein migrates as 66-70 kDa in SDS-PAGE resulting from glycosylation.
• Endotoxin: Less than 1.0 EU per 1 µg of the rhVitronectin by the LAL method.
• Purity: >90% as determined by SDS-PAGE. All lots are greater than 90% pure.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhTNFa should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: VTN vitronectin [ Homo sapiens ]
• Official Symbol: VTN
• Synonyms: VTN; vitronectin; vitronectin (serum spreading factor, somatomedin B, complement S protein); complement S protein; serum spreading factor; somatomedin B; VN; epibolin; S-protein; complement S-protein; serum-spreading factor; V75; VNT
• Gene ID: 7448
• mRNA Refseq: NM_000638
• Protein Refseq: NP_000629
• MIM: 193190
• UniProt ID: P04004