Recombinant Mouse ABCB4 Protein (352-708 aa), GST-tagged

• Cat.No.: ABCB4-301M
• Product Overview: Recombinant Mouse ABCB4 Protein (352-708 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi between hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:8106172, PubMed:7912658, PubMed:7592705, PubMed:7814632, PubMed:8725158, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (By similarity). Required for proper phospholipid bile formation (PubMed:8106172). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:7814632, PubMed:8725158). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (PubMed:1990275).
• Source: E. coli
• Species: Mouse
• Tag: GST
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 66.6 kDa
• Protein length: 352-708 aa
• AA Sequence: DAFANARGAAYVIFDIIDNNPKIDS FSERGHKPDNIKGNLEFSDVHFSYP SRANIKILKGLNLKVKSGQTVALVG NSGCGKSTTVQLLQRLYDPTEGKIS IDGQDIRNFNVRCLREIIGVVSQEP VLFSTTIAENIRYGRGNVTMDEIEK AVKEANAYDFIMKLPQKFDTLVGDR GAQLSGGQKQRIAIARALVRNPKIL LLDEATSALDTESEAEVQAALDKAR EGRTTIVIAHRLSTIRNADVIAGFE DGVIVEQGSHSELMKKEGIYFRLVN MQTAGSQILSEEFEVELSDEKAAGD VAPNGWKARIFRNSTKKSLKSPHQN RLDEETNELDANVPPVSFLKVLKLN KTEWPYF
• Purity: > 90% as determined by SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Abcb4 ATP-binding cassette, sub-family B (MDR/TAP), member 4 [ Mus musculus ]
• Official Symbol: ABCB4
• Synonyms: ABCB4; P glycoprotein 2; P-glycoprotein 3; Mdr2; Pgy2; Pgy-2; mdr-2
• Gene ID: 18670
• mRNA Refseq: NM_008830
• Protein Refseq: NP_032856