Active Recombinant Mouse EphA2 protein

• Cat.No.: Epha2-1722M
• Product Overview: Recombinant Mouse EphA2 protein(Met1-Asn535) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N/A
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Recombinant Mouse EphA2 Protein (ECD) at 2 μg/ml (100 μl/well) can bind Recombinant Human Ephrin-A1 / EFNA1 Protein (Fc Tag), The EC50 is 1.8-5.4 ng/mL.
• Molecular Mass: The recombinant Mouse EphA2 consists of 516 amino acids and predicts a molecular mass of 57.03 kDa. It migrates as an approximately 61.90 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Asn535
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epha2 Eph receptor A2 [ Mus musculus ]
• Official Symbol: EphA2
• Synonyms: EPHA2; Eph receptor A2; ephrin type-A receptor 2; epithelial cell kinase; tyrosine-protein kinase receptor ECK; tyrosine-protein kinase receptor MPK-5; tyrosine-protein kinase receptor SEK-2; Eck; Myk2; Sek2; Sek-2; AW545284
• Gene ID: 13836
• mRNA Refseq: NM_010139
• Protein Refseq: NP_034269