Recombinant Mouse GHR protein(Met1-Gln273), His&hFc-tagged

• Cat.No.: GHR-2359M
• Product Overview: Recombinant Mouse GHR (NP_034414.2)(Met 1-Gln 273) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Gln273
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2 μg/mL in the presence of 50 ng/mL human growth hormone.
• Molecular Mass: The recombinant mouse GHR/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 56.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmGHR/Fc monomer is approximately 70-80 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ghr growth hormone receptor [ Mus musculus ]
• Official Symbol: GHR
• Synonyms: GHR; growth hormone receptor; GH receptor; somatotropin receptor; growth hormone receptor/binding protein; Growth hormone receptor precursor (GH receptor) (GH binding protein) (GHBP) (Serum binding protein); GHBP; GHR/BP; AA986417
• Gene ID: 14600
• mRNA Refseq: NM_001048147
• Protein Refseq: NP_001041612