| Description : |
Interleukin-10 (IL-10), initially known as Cytokine Synthesis Inhibitory Factor (CSIF), belongs to the IL-10 family and shares more than 80% sequence homology with the Epstein-Barr Virus protein BCRFI. It is produced by many immune cells, such as T-cells, macrophages, mast cells and dendritic cells. It is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF, by activated macrophages and Th2 cells. It also displays the ability to suppress Antigen-Presenting Cell (APC) function. The net effect of Interleukin-10 appears to be inhibitory; however, stimulatory effects, such as stimulation of B cell maturation and antibody production, are also reported. |
| Source : |
CHO |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 0.2 ng/mL, measured in a cell proliferation assay using MC/9 cells. |
| Molecular Mass : |
21-23 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
SRGQYSREDNNCTHFPVGQSHMLLE LRTAFSQVKTFFQTKDQLDNILLTD SLMQDFKGYLGCQALSEMIQFYLVE VMPQAEKHGPEIKEHLNSLGEKLKT LRMRLRRCHRFLPCENKSKAVEQVK SDFNKLQDQGVYKAMNEFDIFINCI EAYMMIKMKS |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant murine Interleukin-10 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, murine Interleukin-10 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
