Active Recombinant Mouse Il15ra Protein, Fc-tagged

• Cat.No.: Il15ra-360M
• Product Overview: Recombinant Mouse IL-15 R alpha protein (Gly33 - Lys205) with a Human IgG1 Fc tag (Pro100 - Lys330) at C-terminus was expressed in Mouse myeloma cell line. Contains a small amount (10%) of free IL-15 R alpha and Fc generated by proteolytic cleavage.

Specification

• Description: This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.1-0.5 ng/mL in the presence of 0.1 ng/mL recombinant human IL-15.
• Molecular Mass: 44.9 kDa
• N-terminal Sequence Analysis: Gly33
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Il15ra interleukin 15 receptor, alpha chain [ Mus musculus (house mouse) ]
• Official Symbol: Il15ra
• Synonyms: Il15ra; interleukin 15 receptor, alpha chain; IL-15RA; AA690181; interleukin-15 receptor subunit alpha; IL-15 receptor subunit alpha; IL-15R-alpha
• Gene ID: 16169
• mRNA Refseq: NM_008358
• Protein Refseq: NP_032384
• UniProt ID: Q60819