| Description : |
IL-1 alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. |
| Source : |
E. coli |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.002 ng/mL, corresponding to a specific activity of > 5.0 × 10^8 units/mg. |
| Molecular Mass : |
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| Protein Length : |
156 |
| AA Sequence : |
SAPYTYQSDLRYKLMKLVRQKFVMN DSLNQTIYQDVDKHYLSTTWLNDLQ QEVKFDMYAYSSGGDDSKYPVTLKI SDSQLFVSAQGEDQPVLLKELPETP KLITGSETDLIFFWKSINSKNYFTS AAYPELFIATKEQSRVHLARGLPSM TDFQIS |
| Endotoxin : |
Less than 1 EU/mg of rMuIL-1 alpha as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
