||Recombinant Human IL-1 alpha/IL1A/IL1F1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser113-Ala271) of human IL-1 alpha/IL1A/IL1F1 (Accession #NP_000566.3) fused with a 6×His tag at the C-terminus.
||Interleukin 1 alpha (IL-1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases.
IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.
||Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol.
||< 1.0 EU per μg of the protein as determined by the LAL method.
||> 90% as determined by SDS-PAGE
||Samples are stable for up to twelve months from date of receipt at -70ºC.
||Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.