Active Recombinant Mouse Il1b Protein
Cat.No. : | Il1b-102M |
Product Overview : | Purified recombinant protein of Mouse interleukin 1 beta (Il1b) (Val118-Ser269) without tag was expressed in E.coli. |
- Specification
- Gene Information
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Description : | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. |
Source : | E. coli |
Species : | Mouse |
Bio-activity : | The ED50 is 0.001 -0.005 ng/ml, corresponding to a specific activity of 1- 0.2 x 10^9 units/mg, determined by the dose dependent stimulation of D10S cells proliferation |
Molecular Mass : | 17.4 kDa |
Endotoxin : | Less than 0.01 ng per µg protein as determined by the LAL method |
Purity : | >98%, as determined by Coomassie stained SDS-PAGE. |
Stability : | Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Storage : | Store at -80 centigrade. |
Concentration : | Protein formulated and vialed at >50 ug/mL in specified buffer |
Storage Buffer : | 10mM NaH2PO4, 150mM NaCl, pH7.2 |
Gene Name : | Il1b interleukin 1 beta [ Mus musculus (house mouse) ] |
Official Symbol : | Il1b |
Synonyms : | Il1b; interleukin 1 beta; Il-1b; IL-1beta; interleukin-1 beta; IL-1 beta |
Gene ID : | 16176 |
mRNA Refseq : | NM_008361 |
Protein Refseq : | NP_032387 |
UniProt ID : | P10749 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionInflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.
IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.
Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.
Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.
ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.
IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.
Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.
IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.
CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.
Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.
Customer Reviews (3)
Write a reviewEffective in inflammation studies.
High specificity.
Suitable for cytokine assays.
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