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Recombinant Porcine Interleukin-1 beta

Cat.No. : IL1β-82P
Product Overview : Recombinant Porcine Interleukin-1b produced inE.Coliis a non-glycosylated, Polypeptide chain containing 154 amino acids and having a molecular mass of 17690 Dalton. The IL-1b is purified by proprietary chromatographic techniques.
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  • Gene Information
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Cat. No. : IL-1beta-82P
Description : Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Source : Escherichia Coli.
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Asn-Val-Gln.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized with no additives.
Solubility : It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Protein Content : Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1b as a Reference Standard.
Stability : Lyophilized Interleukin 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL1b should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Pathways : Alzheimer"s disease; Apoptosis; Chagas disease; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Graft-versus-host disease; Hematopoietic cell lineage; Leishmaniasis; MAPK signaling pathway; Malaria; NOD-like receptor signaling pathway; Prion diseases; Toll-like receptor signaling pathway; Type I diabetes mellitus
Gene Name : IL-1beta interleukin-1 beta [ Sus scrofa ]
Synonyms : IL-1beta; interleukin-1 beta; interleukin-1 beta
Gene ID : 396565
mRNA Refseq : NM_001005149
Protein Refseq : NP_001005149
UniProt ID : Q29082

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does IL-1β initiate inflammasome assembly, and which experimental techniques unravel this intricate process? 07/30/2022

Inflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.

What are the specific mechanisms underlying IL-1β's priming of immune cells, and how can flow cytometry and gene expression analyses decipher these molecular pathways? 07/08/2021

IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.

How do post-translational modifications regulate IL-1β's biological activity, and how can mass spectrometry-based phosphoproteomics provide insights into these modifications? 06/06/2020

Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.

How can advanced bioinformatics tools be applied to multi-omics data to comprehensively understand the intricate roles of IL-1β in health and disease? 04/16/2020

Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.

What is the significance of IL-1β's epigenetic regulation in chronic inflammatory diseases, and how can ChIP-seq and DNA methylation profiling uncover these regulatory elements? 11/01/2019

ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.

How does IL-1β interact with its downstream effectors to induce the production of pro-inflammatory cytokines, and how can dual-luciferase reporter assays reveal these intricate interactions? 03/31/2019

IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.

What are the complex feedback loops between IL-1β and other cytokines, and how can systems biology approaches like network analysis dissect these dynamic interactions? 01/24/2019

Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.

What is the role of IL-1β in modulating the tumor microenvironment, and how can advanced imaging techniques like intravital microscopy elucidate its impact on immune cell dynamics? 08/08/2017

IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.

What are the mechanisms underlying IL-1β's non-canonical signaling pathways, and how can CRISPR-Cas9 screening identify key mediators of these alternative pathways? 04/18/2017

CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.

How does IL-1β's signaling adapt to different cellular contexts, and how can single-cell RNA sequencing uncover cell-specific responses to this cytokine? 01/03/2017

Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.

Customer Reviews (3)

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Reviews
11/08/2021

    Effective in inflammation studies.

    04/23/2021

      High specificity.

      06/30/2020

        Suitable for cytokine assays.

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