Recombinant Porcine Interleukin-1 beta
Cat.No. : | IL1β-82P |
Product Overview : | Recombinant Porcine Interleukin-1b produced inE.Coliis a non-glycosylated, Polypeptide chain containing 154 amino acids and having a molecular mass of 17690 Dalton. The IL-1b is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
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Cat. No. : | IL-1beta-82P |
Description : | Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
Source : | Escherichia Coli. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Asn-Val-Gln. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein was lyophilized with no additives. |
Solubility : | It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1b as a Reference Standard. |
Stability : | Lyophilized Interleukin 1b although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL1b should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Pathways : | Alzheimer"s disease; Apoptosis; Chagas disease; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Graft-versus-host disease; Hematopoietic cell lineage; Leishmaniasis; MAPK signaling pathway; Malaria; NOD-like receptor signaling pathway; Prion diseases; Toll-like receptor signaling pathway; Type I diabetes mellitus |
Gene Name : | IL-1beta interleukin-1 beta [ Sus scrofa ] |
Synonyms : | IL-1beta; interleukin-1 beta; interleukin-1 beta |
Gene ID : | 396565 |
mRNA Refseq : | NM_001005149 |
Protein Refseq : | NP_001005149 |
UniProt ID : | Q29082 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionInflammasome assembly initiated by IL-1β is deciphered using techniques like co-immunoprecipitation, proximity ligation assays, and super-resolution microscopy.
IL-1β's priming of immune cells is dissected through flow cytometry, RNA sequencing, and chromatin immunoprecipitation, revealing its epigenetic and transcriptional effects.
Mass spectrometry-based phosphoproteomics deciphers IL-1β's post-translational modifications, revealing regulatory sites crucial for its activation and signaling.
Bioinformatics tools integrate multi-omics data to provide a comprehensive understanding of IL-1β's roles, revealing its intricate contributions to health and disease.
ChIP-seq and DNA methylation profiling uncover IL-1β's epigenetic impact on target genes, shedding light on its role in shaping chronic inflammatory states.
IL-1β's interactions with downstream effectors are unveiled using dual-luciferase reporter assays, surface plasmon resonance, and co-crystallization studies.
Network analysis integrates omics data to unravel IL-1β's intricate cross-talk with other cytokines, mapping out complex regulatory loops in inflammation.
IL-1β's impact on the tumor microenvironment is studied using intravital microscopy, 3D culture models, and single-cell RNA sequencing to capture dynamic cell behavior.
CRISPR-Cas9 screening identifies IL-1β's non-canonical signaling mediators through functional genomics, revealing alternative pathways in cellular responses.
Single-cell RNA sequencing unveils IL-1β's context-specific responses, exposing cell type-specific signaling and gene expression patterns.
Customer Reviews (3)
Write a reviewEffective in inflammation studies.
High specificity.
Suitable for cytokine assays.
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