Recombinant Mouse Lep Protein

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Recombinant Mouse Lep Protein

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Cat.No. : Lep-120M
Product Overview : Purified recombinant protein of Mouse leptin (Lep) without tag was expressed in E.coli.
Description : Biased expression in subcutaneous fat pad adult (RPKM 267.7), genital fat pad adult (RPKM 183.5) and 1 other tissue.
Source : E. coli
Species : Mouse
Bio-activity : PeproTech's murine Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 ug Leptin/gm of body weight for 14 days. Significant effects on body weight , food consumption, and plasma glucose levels were observed to saline-treated controls
Molecular Mass : 16.2 kDa
AA Sequence : MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
Endotoxin : Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
Purity : >95%, as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Storage : Store at -80 centigrade.
Storage Buffer : Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
Reconstitution : Resuspend the protein in the desired concentration in proper buffer.
Figure :

Gene Name : Lep leptin [ Mus musculus (house mouse) ]
Official Symbol : Lep
Synonyms : Lep; leptin; ob; obese; leptin; obesity factor
Gene ID : 16846
mRNA Refseq : NM_008493
Protein Refseq : NP_032519
UniProt ID : P41160
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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