Recombinant Mouse Lep protein(Met1-Cys167), mFc-tagged

• Cat.No.: Lep-2367M
• Product Overview: Recombinant Mouse Leptin (P41160) (Met1-Cys167) was expressed in HEK293 with the Fc region of Mouse IgG2a at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: Met1-Cys167
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse Leptin consists of 394 amino acids and predicts a molecular mass of 43.33 kDa. It migrates as an approximately 50.13 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lep leptin [ Mus musculus ]
• Official Symbol: Lep
• Synonyms: LEP; leptin; obesity factor; ob; obese
• Gene ID: 16846
• mRNA Refseq: NM_008493
• Protein Refseq: NP_032519