Recombinant Mouse LOXL2 Protein (26-776 aa), His-tagged

• Cat.No.: LOXL2-630M
• Product Overview: Recombinant Mouse LOXL2 Protein (26-776 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in Extracellular domain matrix, promotes cross-linking of Extracellular domain matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (T) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3 . Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.1 Publication.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 88.4 kDa
• Protein length: 26-776 aa
• AA Sequence: QYEGWPYQLQYPEYFQQPAPEHHQR QVPSDVVKIQVRLAGQKRKHNEGRV EVYYEGQWGTVCDDDFSIHAAHVVC RQVGYVEAKSWAASSSYGPGEGPIW LDNIYCTGKESTLASCSSNGWGVTD CKHTEDVGVVCSEKRIPGFKFDNSL INQIESLNIQVEDIRIRPILSAFRH RKPVTEGYVEVKEGKAWKQICNKHW TAKNSHVVCGMFGFPAEKTYNPKAY KTFASRRKLRYWKFSMNCTGTEAHI SSCKLGPSVTRDPVKNATCENGQPA VVSCVPSQIFSPDGPSRFRKAYKPE QPLVRLRGGAQVGEGRVEVLKNGEW GTICDDKWDLVSASVVCRELGFGTA KEAITGSRLGQGIGPIHLNEVQCTG TEKSIIDCKFNTESQGCNHEEDAGV RCNIPIMGFQKKVRLNGGRNPYEGR VEVLTERNGSLVWGTVCGQNWGIVE AMVVCRQLGLGFASNAFQETWYWHG NIFANNVVMSGVKCSGTELSLAHCR HDEEVACPEGGVRFGAGVACSETAP DLVLNAEIVQQTAYLEDRPMSLLQC AMEENCLSASAVHTDPTRGHRRLLR FSSQIHNNGQSDFRPKNGRHAWIWH DCHRHYHSMEVFTYYDLLSLNGTKV AEGHKASFCLEDTECEGDIQKSYEC ANFGEQGITMGCWDMYRHDIDCQWI DITDVPPGDYLFQVVINPNYEVPES DFSNNIMKCRSRYDGYRIWMYNCHV GGAFSEETEQKFEHFSGLLNNQLSV Q
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Loxl2 lysyl oxidase-like 2 [ Mus musculus ]
• Official Symbol: LOXL2
• Synonyms: LOXL2; 1110004B06Rik; 4930526G11Rik; 9430067E15Rik; MGC102220
• Gene ID: 94352
• mRNA Refseq: NM_033325
• Protein Refseq: NP_201582
• UniProt ID: P58022

Reviews

06/20/2022

This protein exhibits outstanding performance in ELISA, providing accurate and reliable results in detecting and quantifying specific antigens.

01/16/2022

Its high sensitivity ensures the detection of even low abundance targets, making it a valuable tool in immunoassays.

03/23/2016

Its stability and compatibility with electron microscopy techniques make it an excellent choice for studying protein structures at high resolution.

Q&As

In what ways does LOXL2 impact tissue elasticity, and how is this relevant in clinical applications?

LOXL2 promotes the cross-linking of elastin fibers, affecting tissue elasticity. Understanding these mechanisms can have implications in diseases where tissue flexibility is crucial, such as in cardiovascular conditions.

How does LOXL2 influence wound healing, and can it be targeted for therapeutic interventions?

LOXL2 is involved in tissue repair and wound healing by modulating the extracellular matrix. Targeting LOXL2 may offer therapeutic opportunities to enhance or control the wound healing process.

Are there diagnostic applications of LOXL2 in clinical settings?

Elevated levels of LOXL2 have been associated with certain diseases. Measuring LOXL2 levels can potentially serve as a diagnostic marker for conditions such as liver fibrosis and certain cancers.

Are there ongoing clinical trials targeting LOXL2 for therapeutic purposes?

Yes, several clinical trials are exploring the potential of LOXL2 inhibitors in treating fibrosis and cancer. These trials aim to validate the efficacy and safety of targeting LOXL2 in clinical settings.

How does LOXL2 contribute to the clinical understanding of fibrosis?

LOXL2 is implicated in fibrosis as it plays a key role in collagen cross-linking, influencing tissue stiffness and architecture, making it a potential target for anti-fibrotic therapies.