Recombinant Mouse Napepld Protein, Myc/DDK-tagged

• Cat.No.: Napepld-003M
• Product Overview: Purified recombinant protein of Mouse N-acyl phosphatidylethanolamine phospholipase D (Napepld), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid (PubMed:14634025, PubMed:15760304, PubMed:17655883, PubMed:21801852). Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain (PubMed:21801852, PubMed:16605240). May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation (PubMed:31685899). As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose (PubMed:25757720). Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs (PubMed:14634025).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 45.8 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Napepld N-acyl phosphatidylethanolamine phospholipase D [ Mus musculus (house mouse) ]
• Official Symbol: Napepld
• Synonyms: Napepld; N-acyl phosphatidylethanolamine phospholipase D; NAP; Mbldc1; NAPE-PLD; A530089G06; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; Metallo-beta-lactamase domain containing 1; NAPE-hydrolyzing phospholipase D; EC 3.1.4.54
• Gene ID: 242864
• mRNA Refseq: NM_178728
• Protein Refseq: NP_848843
• UniProt ID: Q8BH82