Recombinant Mouse Podxl Protein, Myc/DDK-tagged

• Cat.No.: Podxl-4977M
• Product Overview: Purified recombinant protein of mouse full-length podocalyxin-like (Podxl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
• Molecular Mass: 53.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Podxl podocalyxin-like [ Mus musculus (house mouse) ]
• Official Symbol: Podxl
• Synonyms: PODXL; podocalyxin-like; podocalyxin; podocalyxin-like protein 1; PC; Ly102; Pclp1; PCLP-1; AW121214
• Gene ID: 27205
• mRNA Refseq: NM_013723
• Protein Refseq: NP_038751
• UniProt ID: Q9R0M4