Recombinant Mouse Sparc Protein, His-tagged

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Recombinant Mouse Sparc Protein, His-tagged

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Cat.No. : Sparc-034M
Product Overview : Recombinant mouse SPARC, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description : Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Source : Insect cells
Species : Mouse
Tag : His
Form : Liquid
Molecular Mass : 33.3 kDa
AA Sequence : APQQTEVAEE IVEEETVVEE TGVPVGANPV QVEMGEFEDG AEETVEEVVA DNPCQNHHCK HGKVCELDES NTPMCVCQDP TSCPAPIGEF EKVCSNDNKT FDSSCHFFAT KCTLEGTKKG HKLHLDYIGP CKYIAPCLDS ELTEFPLRMR DWLKNVLVTL YERDEGNNLL TEKQKLRVKK IHENEKRLEA GDHPVELLAR DFEKNYNMYI FPVHWQFGQL DQHPIDGYLS HTELAPLRAP LIPMEHCTTR FFETCDLDND KYIALEEWAG CFGIKEQDIN KDLVI<HHHH HH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 95% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -113 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by Absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Gene Name : Sparc secreted acidic cysteine rich glycoprotein [ Mus musculus (house mouse) ]
Official Symbol : Sparc
Synonyms : Sparc; secreted acidic cysteine rich glycoprotein; ON; BM-40; osteo; SPARC; basement-membrane protein 40; osteonectin; secreted protein acidic and rich in cysteine
Gene ID : 20692
mRNA Refseq : NM_009242
Protein Refseq : NP_033268
UniProt ID : P07214

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