Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human SPARC protein, His-tagged

Cat.No. : SPARC-102H
Product Overview : Recombinant Human SPARC protein, fused to His-tag and was expressed in E.coli.
  • Specification
  • Gene Information
  • Related Products
Description : This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
Source : E.coli
Species : Human
Tag : His
Form : Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 μg/mL, corresponding to a specific activity of > 333 IU/mg.
Molecular Mass : Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 x His tag).
Protein length : 314
AA Sequence : MSYYHHHHHHDYDIPTTENLYFQGA MGSAPQQEALPDETEVVEETVAEVT EVSVGANPVQVEVGEFDDGAEETEE EVVAENPCQNHHCKHGKVCELDENN TPMCVCQDPTSCPAPIGEFEKVCSN DNKTFDSSCHFFATKCTLEGTKKGH KLHLDYIGPCKYIPPCLDSELTEFP LRMRDWLKNVLVTLYERDEDNNLLT EKQKLRVKKIHENEKRLEAGDHPVE LLARDFEKNYNMYIFPVHWQFGQLD QHPIDGYLSHTELAPLRAPLIPMEH CTTRFFETCDLDNDKYIALDEWAGC FGIKQKDIDKDLVI
Endotoxin : Less than 1 EU/μg of rHuSPARC, His as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analyses.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : SPARC
Official Symbol : SPARC
Synonyms : ON; OI17; BM-40
Gene ID : 6678
mRNA Refseq : NM_003118.4
Protein Refseq : NP_003109.1
MIM : 182120
UniProt ID : P09486

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
How is the SPARC protein linked to cancer? 04/18/2023

SPARC is associated with cancer progression, as it can influence tumor growth, invasion, and angiogenesis. Its expression levels in tumors are often studied for prognostic and diagnostic purposes.

Can SPARC be a potential therapeutic target for cancer treatment? 12/08/2022

Yes, SPARC is being explored as a therapeutic target for cancer. Researchers are investigating ways to modulate its activity to hinder tumor progression or enhance the efficacy of existing treatments.

In what ways can SPARC expression be detected and measured in clinical settings? 04/25/2021

SPARC expression can be detected through techniques like immunohistochemistry, Western blotting, and molecular assays. These methods help assess SPARC levels in tissues or blood samples.

How does SPARC contribute to tissue repair and regeneration? 03/04/2021

SPARC plays a role in tissue repair by influencing cell-matrix interactions and modulating the extracellular environment. Its involvement in wound healing and tissue regeneration makes it a subject of interest in regenerative medicine.

Are there any ongoing clinical trials targeting SPARC for therapeutic interventions? 03/28/2019

Yes, there are clinical trials exploring the potential of targeting SPARC in various diseases, including cancer. These trials aim to evaluate the safety and efficacy of SPARC-modulating agents.

Customer Reviews (3)

Write a review
Reviews
07/18/2021

    The SPARC protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.

    02/09/2017

      Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.

      07/10/2016

        Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.

        Ask a Question for All SPARC Products

        Required fields are marked with *

        My Review for All SPARC Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends