Recombinant Human SPARC protein, His-tagged
Cat.No. : | SPARC-102H |
Product Overview : | Recombinant Human SPARC protein, fused to His-tag and was expressed in E.coli. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. |
Source : | E.coli |
Species : | Human |
Tag : | His |
Form : | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 μg/mL, corresponding to a specific activity of > 333 IU/mg. |
Molecular Mass : | Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 x His tag). |
Protein length : | 314 |
AA Sequence : | MSYYHHHHHHDYDIPTTENLYFQGA MGSAPQQEALPDETEVVEETVAEVT EVSVGANPVQVEVGEFDDGAEETEE EVVAENPCQNHHCKHGKVCELDENN TPMCVCQDPTSCPAPIGEFEKVCSN DNKTFDSSCHFFATKCTLEGTKKGH KLHLDYIGPCKYIPPCLDSELTEFP LRMRDWLKNVLVTLYERDEDNNLLT EKQKLRVKKIHENEKRLEAGDHPVE LLARDFEKNYNMYIFPVHWQFGQLD QHPIDGYLSHTELAPLRAPLIPMEH CTTRFFETCDLDNDKYIALDEWAGC FGIKQKDIDKDLVI |
Endotoxin : | Less than 1 EU/μg of rHuSPARC, His as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analyses. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | SPARC |
Official Symbol : | SPARC |
Synonyms : | ON; OI17; BM-40 |
Gene ID : | 6678 |
mRNA Refseq : | NM_003118.4 |
Protein Refseq : | NP_003109.1 |
MIM : | 182120 |
UniProt ID : | P09486 |
Products Types
◆ Recombinant Protein | ||
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Sparc-034M | Recombinant Mouse Sparc Protein, His-tagged | +Inquiry |
Sparc-010M | Recombinant Mouse Sparc Protein, His-tagged | +Inquiry |
Sparc-6073M | Recombinant Mouse Sparc Protein, Myc/DDK-tagged | +Inquiry |
◆ Native Protein | ||
SPARC-30653TH | Native Human SPARC | +Inquiry |
SPARC-287B | Native Bovine Osteonectin | +Inquiry |
◆ Lysates | ||
SPARC-2120MCL | Recombinant Mouse SPARC cell lysate | +Inquiry |
SPARC-2616HCL | Recombinant Human SPARC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionSPARC is associated with cancer progression, as it can influence tumor growth, invasion, and angiogenesis. Its expression levels in tumors are often studied for prognostic and diagnostic purposes.
Yes, SPARC is being explored as a therapeutic target for cancer. Researchers are investigating ways to modulate its activity to hinder tumor progression or enhance the efficacy of existing treatments.
SPARC expression can be detected through techniques like immunohistochemistry, Western blotting, and molecular assays. These methods help assess SPARC levels in tissues or blood samples.
SPARC plays a role in tissue repair by influencing cell-matrix interactions and modulating the extracellular environment. Its involvement in wound healing and tissue regeneration makes it a subject of interest in regenerative medicine.
Yes, there are clinical trials exploring the potential of targeting SPARC in various diseases, including cancer. These trials aim to evaluate the safety and efficacy of SPARC-modulating agents.
Customer Reviews (3)
Write a reviewThe SPARC protein finds great utility in protein electron microscopy structure analysis, consistently delivering exceptional results.
Its remarkable sensitivity and specificity make it a valuable tool for accurate and robust data analysis, bolstering the credibility of research outcomes.
Notably, it exhibits exceptional performance in ELISA assays, providing reliable and precise results in the detection and quantification of target molecules.
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