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Recombinant Pig IL1B Protein

Cat.No.: IL1B-1024P
Product Overview: Recombinant sus scrofa IL1B (NP_999220.1) (Ala115-Pro267) was expressed with an initial Met.
Source: E. coli
Species: Pig
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED50 for this effect is typically 0.08-0.4 ng/mL.
Molecular Mass: The recombinant sus scrofa IL1B consists 154 amino acids and predicts a molecular mass of 17.7 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: IL1B interleukin 1, beta [ Sus scrofa ]
Official Symbol: IL1B
Synonyms: IL1B; interleukin 1, beta; interleukin-1 beta; IL-1 beta; prointerleukin-1 beta;
Gene ID: 397122
mRNA Refseq: NM_214055
Protein Refseq: NP_999220
MIM:
UniProt ID:
Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved bi

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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