Recombinant Rat Cd86 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd86-4087RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rat Cd86 (NP_064466.1) extracellular domain (Met 1-Ile 250), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant rat CD86 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. The apparent molecular mass of the rat CD86 is approximately 40-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: 232
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd86 CD86 molecule [ Rattus norvegicus ]
• Official Symbol: Cd86
• Gene ID: 56822
• mRNA Refseq: NM_020081
• Protein Refseq: NP_064466

Reviews

01/09/2022

With the CD86 protein's high-quality composition and reliability, researchers can confidently proceed with their studies, knowing that they have access to a protein that consistently delivers accurate results.

05/30/2021

Its high purity and stability ensure reliable and reproducible results, which are paramount for achieving meaningful scientific outcomes.

12/08/2017

The CD86 protein is renowned for its outstanding quality, making it an optimal choice to fulfill the requirements of my experimental investigations.

Q&As

Are there any ongoing clinical trials involving CD86 inhibitors?

Yes, several clinical trials are investigating the efficacy of CD86 inhibitors in various diseases, including autoimmune disorders and certain cancers.

How does CD86 contribute to transplant medicine?

In transplantation, CD86 blockade is investigated as a potential strategy to modulate the immune response and prevent graft rejection by inhibiting excessive T cell activation.

How does CD86 influence the balance between Th1 and Th2 immune responses?

CD86 signaling is involved in the differentiation of T helper cells. Modulating CD86 levels may influence the balance between Th1 and Th2 responses, which is relevant in various immune-mediated diseases.

Can CD86 be used as a prognostic marker in certain diseases?

There is emerging evidence suggesting that CD86 expression levels may have prognostic value in certain diseases, guiding clinicians in predicting disease outcomes.

How does CD86 influence the development of regulatory T cells (Tregs)?

CD86 signaling is involved in the development and function of regulatory T cells, which play a crucial role in maintaining immune tolerance and preventing autoimmune reactions.