Recombinant Human CD86 cell lysate

• Cat.No.: CD86-2619HCL
• Product Overview: Human CD86 / B7-2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CD86 CD86 molecule [ Homo sapiens ]
• Official Symbol: CD86
• Synonyms: CD86; CD86 molecule; CD28LG2, CD86 antigen (CD28 antigen ligand 2, B7 2 antigen); T-lymphocyte activation antigen CD86; B lymphocyte antigen B7 2; B7 2; B7.2; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); B70; B7-2; LAB72; CD28LG2; MGC34413
• Gene ID: 942
• mRNA Refseq: NM_001206924
• Protein Refseq: NP_001193853
• MIM: 601020
• UniProt ID: P42081
• Chromosome Location: 3q21
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem
• Function: coreceptor activity; protein binding; receptor activity

Reviews

01/09/2022

With the CD86 protein's high-quality composition and reliability, researchers can confidently proceed with their studies, knowing that they have access to a protein that consistently delivers accurate results.

05/30/2021

Its high purity and stability ensure reliable and reproducible results, which are paramount for achieving meaningful scientific outcomes.

12/08/2017

The CD86 protein is renowned for its outstanding quality, making it an optimal choice to fulfill the requirements of my experimental investigations.

Q&As

Are there any ongoing clinical trials involving CD86 inhibitors?

Yes, several clinical trials are investigating the efficacy of CD86 inhibitors in various diseases, including autoimmune disorders and certain cancers.

How does CD86 contribute to transplant medicine?

In transplantation, CD86 blockade is investigated as a potential strategy to modulate the immune response and prevent graft rejection by inhibiting excessive T cell activation.

How does CD86 influence the balance between Th1 and Th2 immune responses?

CD86 signaling is involved in the differentiation of T helper cells. Modulating CD86 levels may influence the balance between Th1 and Th2 responses, which is relevant in various immune-mediated diseases.

Can CD86 be used as a prognostic marker in certain diseases?

There is emerging evidence suggesting that CD86 expression levels may have prognostic value in certain diseases, guiding clinicians in predicting disease outcomes.

How does CD86 influence the development of regulatory T cells (Tregs)?

CD86 signaling is involved in the development and function of regulatory T cells, which play a crucial role in maintaining immune tolerance and preventing autoimmune reactions.