| Description : |
Interleukin-1 alpha (IL-1α), is produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including stimulation of thymocyte proliferation via IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. |
| Source : |
CHO |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 5 pg/mL, measured in a proliferation assay using D10S cells. |
| Molecular Mass : |
17~22 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
APHSFQNNLRYKLIRIVKQEFIMND SLNQNIYVDMDRIHLKAASLNDLQL EVKFDMYAYSSGGDDSKYPVTLKVS NTQLFVSAQGEDKPVLLKEIPETPK LITGSETDLIFFWEKINSKNYFTSA AFPELLIATKEQSQVHLARGLPSMI DFQIS |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant Rat Interleukin-1 alpha remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Rat Interleukin-1 alpha should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
