Recombinant Rat IL21 cell lysate

• Cat.No.: IL21-001RCL
• Product Overview: Rat IL21 / Interleukin 21 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Rat
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Il21 interleukin 21 [ Rattus norvegicus ]
• Official Symbol: IL21
• Synonyms: IL21; interleukin 21; interleukin-21; IL-21
• Gene ID: 365769
• mRNA Refseq: NM_001108943
• Protein Refseq: NP_001102413
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; cytokine receptor binding; interleukin-2 receptor binding; interleukin-2 receptor binding; molecular_function

Reviews

08/19/2022

With the IL21 protein at hand, I am excited to embark on my scientific journey with confidence and optimism.

01/30/2022

I am confident that incorporating the IL21 protein into my research will yield accurate and reliable results, enabling deeper insights into cellular processes

04/23/2019

Their expertise and guidance will undoubtedly prove valuable in overcoming hurdles and maximizing the potential of this protein in my research endeavors.

Q&As

In which types of cancer has IL21 protein shown promising results?

IL21 has demonstrated efficacy in preclinical and clinical studies for various cancers, including melanoma, renal cell carcinoma, and lymphoma.

Are there any side effects associated with IL21 therapy?

Common side effects may include fatigue, fever, and flu-like symptoms. However, the severity and type of side effects can vary among individuals.

Can IL21 be used as a standalone treatment for cancer?

IL21 is often investigated in combination with other cancer therapies to enhance its effectiveness, and it has shown promise in combination treatments.

Are there any ongoing clinical trials exploring the use of IL21 in autoimmune conditions?

Yes, several clinical trials are investigating the efficacy and safety of IL21 in autoimmune diseases, such as rheumatoid arthritis and lupus.

How does IL21 influence the development of memory T cells?

IL21 promotes the differentiation and survival of memory T cells, contributing to the long-term immune response against pathogens.