Recombinant Rat Ntn1 protein, His/GST-tagged

• Cat.No.: Ntn1-257R
• Product Overview: Recombinant Rat Ntn1(Pro313~Pro565) fused with two N-terminal Tags, His-tag and GST-tag was expressed in E. coli.

Specification

• Description: Ntn1 played an important role in many functions.
• Source: E. coli
• Species: Rat
• Tag: His&GST
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 60.5kDa
• Protein length: Pro313~Pro565
• AA Sequence: PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSMEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIAXKCPKIK PLKKYLLLGN AEDSP
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Ntn1 netrin 1 [ Rattus norvegicus ]
• Official Symbol: Ntn1
• Synonyms: NTN1; netrin 1; netrin-1
• Gene ID: 114523
• mRNA Refseq: NM_053731
• Protein Refseq: NP_446183
• Chromosome Location: 10q24
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; DCC mediated attractive signaling, organism-specific biosystem

Citation

The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats

Journal: Frontiers in Cellular Neuroscience    PubMed ID: 29209172    Data: 2017/11/3

Authors: Liangjie Bai, Xifan Mei, Gang Lv

Article Snippet:Recombinant rat Netrin-1 (Creative BioMart, Shirley, NY, USA) was dissolved in 1× phosphate-buffered saline (PBS) to achieve a final concentration of 800 ng/mL.. Compound C (Selleck Chemicals LLC, USA) was dissolved in 1× PBS and administered at a dose of 20 mg/kg (Bai et al., ).Compound C (Selleck Chemicals LLC, USA) was dissolved in 1× PBS and administered at a dose of 20 mg/kg (Bai et al., ).

Netrin-1-promotes transcription factor EB (TFEB) nuclear translocation via the AMP-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signal pathway after spinal cord injury (SCI). (A) Western blots of p-AMPK, p-Acetyl-CoA Carboxylase (p-ACC), p-mTOR, p-P70S6K, TFEB, H3 and β-tubulin in each group. (B,C,E) Quantification of p-AMPK, p-ACC, p-mTOR, p-P70S6K, TFEB, H3 and β-tubulin in each group ( n = 5). (D) Double staining for NeuN (green)/TFEB (red) of sections from the spinal cord in each group. (F) Quantification of the nuclear-translocation neurons of TFEB in each group ( n = 5); * P < 0.05 vs. SCI group, ** P < 0.01 vs. SCI group, & P < 0.05 vs. Netrin-1 group && P < 0.01 vs. Netrin-1 group.

Netrin-1 enhances lysosomal biogenesis after SCI. (A) Western blots of lysosomal-associated membrane protein 1 (LAMP1), ATP6V1A, CTSD (Pre-CTSD and Mature-CTSD) and β-tubulin in each group. (B,C,E) Quantification of LAMP1, ATP6V1A, CTSD (Pre-CTSD and Mature-CTSD) and β-tubulin in each group ( n = 5). (D) Double staining for NeuN (green)/LAMP1 (red) of sections from the spinal cord in each group. (F) Quantification of the number of LAMP1-positive neurons in each group ( n = 5); * P < 0.05 vs. SCI group, ** P < 0.01 vs. SCI group, & P < 0.05 vs. Netrin-1 group && P < 0.01 vs. Netrin-1 group.

Netrin-1 promotes autophagy flux after SCI. (A) Western blots of light chain 3 (LC3)-I, LC3-II, p62 and β-tubulin in each group. (B,C) Quantification of LC3-I, LC3-II, p62 and β-tubulin in each group ( n = 5). (D) Double staining for NeuN (green)/LC3 (red) of sections from the spinal cord in each group (white arrows: LC3-positive neurons). (E) Quantification of the number of LC3-positive neurons in each group ( n = 5). (F) Double staining for NeuN (green)/p62 (red) of sections from the spinal cord in each group. (G) Quantification of the number of p62-positive neurons in each group ( n = 5). * P < 0.05 vs. SCI group, ** P < 0.01 vs. SCI group; && P < 0.01 vs. Netrin-1 group.

Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury

Journal: Scientific Reports    PubMed ID: 28186165    Data: 2017/2/10

Authors: Liangjie Bai, Xifan Mei, Gang Lv

Article Snippet:Recombinant rat Netrin-1 was purchased from Creative BioMart (Shirley, NY, USA).. Anti-LC3B, anti-Beclin-1, anti-NeuN, anti-mTOR, and anti-p-mTOR antibody as well as goat anti-rabbit and goat anti-mouse-IgG HRP were purchased from Abcam (Cambridge, MA, USA).Anti-LC3B, anti-Beclin-1, anti-NeuN, anti-mTOR, and anti-p-mTOR antibody as well as goat anti-rabbit and goat anti-mouse-IgG HRP were purchased from Abcam (Cambridge, MA, USA).

BBB scores of the sham, SCI, and Netrin-1 groups were evaluated at 0, 1, 3, 7, 14, and 21 days after contusion. *P < 0.05 vs. the SCI group; **P < 0.01 vs. the SCI group. Data represented mean ± SEM (n = 6).

(a) Expression of AMPK, p-AMPK, mTOR, p-mTOR, p-P70S6K, P70S6K, Beclin-1, LC3B, and β-tubulin in the sham, SCI, Netrin-1, Netrin-1+ compound C, and compound C groups. (b,c,d) Quantification analysis of p-mTOR/mTOR p-P70S6K/P70S6K, p-AMPK/AMPK, LC3B-II/LC3B-I, and Beclin-1/β-tubulin in each group. Mean ± SD, n = 3. *P < 0.05 vs. the SCI group; **P < 0.01 vs. the SCI group; & P < 0.05 vs. the Netrin-1+ compound C group; && P < 0.01 vs. the Netrin-1+ compound C group.

(a) Double staining for NeuN (green)/LC3B (red) of sections from the spinal cord sample in the sham, SCI, and Netrin-1 groups. Scale bar = 100 μm (white arrows: LC3B-positive neurons). (b) Quantification of the number of LC3B-positive neurons in each group. Data were represented as mean ± SD, n = 5. **P < 0.01 vs. the SCI group; ## P < 0.01 vs. the sham group.

Publication :

Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury (2017)

Neuroprotection of netrin-1 on neurological recovery via Wnt/β-catenin signaling pathway after spinal cord injury (2020)

The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats (2017)

Reviews

04/26/2022

It has high biological activity, high clearance rate and high safety.

02/03/2021

The storage conditions are broad and do not affect the activity of the protein,

01/27/2019

This protein is very reproducible.

Q&As

Are there have any use of NTN1 protein in cancer immunotherapy?

The targeted effect of NTN1 protein can be used to develop tumor immunotherapy drugs for the immune recognition and killing mechanism of tumors.

What are the applications of NTN1 protein in restorative and regenerative medicine?

NTN1 protein can help with cell migration, proliferation, and directed growth, so it may be a potential therapeutic target for cell therapy and restorative medicine.

In which diseases does NTN1 protein play an important role?

NTN1 protein not only plays an important role in the development of the nervous system, but also closely related to the occurrence and development of various diseases such as nervous system tumors, cardiovascular diseases and inflammatory diseases.

Is the NTN1 protein associated with inflammation?

Yes, NTN1 protein is involved in the regulatory process of inflammatory response and may play an important role in the occurrence and development of inflammatory diseases.

What is the role of NTN1 protein in autoimmune diseases?

This protein is closely related to the occurrence and progression of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus, and may be involved in the regulation of autoimmune responses.

Why does NTN1 protein play a role in transplantation immunity?

NTN1 protein may be involved in the regulation of graft rejection, affecting immune tolerance and treatment efficacy after transplantation. It is also a potential transplant immunotherapy target.