Recombinant Rat Ntn1 protein, His/GST-tagged

• Cat.No.: Ntn1-257R
• Product Overview: Recombinant Rat Ntn1(Pro313~Pro565) fused with two N-terminal Tags, His-tag and GST-tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: Pro313~Pro565
• Description: Ntn1 played an important role in many functions.
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 60.5kDa
• AA Sequence: PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSMEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIAXKCPKIK PLKKYLLLGN AEDSP
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Publications:
  Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury (2017)
  Neuroprotection of netrin-1 on neurological recovery via Wnt/β-catenin signaling pathway after spinal cord injury (2020)
  The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats (2017)

Gene Information

• Gene Name: Ntn1 netrin 1 [ Rattus norvegicus ]
• Official Symbol: Ntn1
• Synonyms: NTN1; netrin 1; netrin-1
• Gene ID: 114523
• mRNA Refseq: NM_053731
• Protein Refseq: NP_446183
• Chromosome Location: 10q24
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; DCC mediated attractive signaling, organism-specific biosystem

Citation

The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats

Journal: Frontiers in Cellular Neuroscience    PubMed ID: 29209172    Data: 2017/11/3

Authors: Liangjie Bai, Xifan Mei, Gang Lv

Article Snippet:Recombinant rat Netrin-1 (Creative BioMart, Shirley, NY, USA) was dissolved in 1× phosphate-buffered saline (PBS) to achieve a final concentration of 800 ng/mL.. Compound C (Selleck Chemicals LLC, USA) was dissolved in 1× PBS and administered at a dose of 20 mg/kg (Bai et al., ).Compound C (Selleck Chemicals LLC, USA) was dissolved in 1× PBS and administered at a dose of 20 mg/kg (Bai et al., ).

Netrin-1-promotes transcription factor EB (TFEB) nuclear translocation via the AMP-activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signal pathway after spinal cord injury (SCI). (A) Western blots of p-AMPK, p-Acetyl-CoA Carboxylase (p-ACC), p-mTOR, p-P70S6K, TFEB, H3 and β-tubulin in each group. (B,C,E) Quantification of p-AMPK, p-ACC, p-mTOR, p-P70S6K, TFEB, H3 and β-tubulin in each group ( n = 5). (D) Double staining for NeuN (green)/TFEB (red) of sections from the spinal cord in each group. (F) Quantification of the nuclear-translocation neurons of TFEB in each group ( n = 5); * P < 0.05 vs. SCI group, ** P < 0.01 vs. SCI group, & P < 0.05 vs. Netrin-1 group && P < 0.01 vs. Netrin-1 group.

Netrin-1 enhances lysosomal biogenesis after SCI. (A) Western blots of lysosomal-associated membrane protein 1 (LAMP1), ATP6V1A, CTSD (Pre-CTSD and Mature-CTSD) and β-tubulin in each group. (B,C,E) Quantification of LAMP1, ATP6V1A, CTSD (Pre-CTSD and Mature-CTSD) and β-tubulin in each group ( n = 5). (D) Double staining for NeuN (green)/LAMP1 (red) of sections from the spinal cord in each group. (F) Quantification of the number of LAMP1-positive neurons in each group ( n = 5); * P < 0.05 vs. SCI group, ** P < 0.01 vs. SCI group, & P < 0.05 vs. Netrin-1 group && P < 0.01 vs. Netrin-1 group.

Netrin-1 promotes autophagy flux after SCI. (A) Western blots of light chain 3 (LC3)-I, LC3-II, p62 and β-tubulin in each group. (B,C) Quantification of LC3-I, LC3-II, p62 and β-tubulin in each group ( n = 5). (D) Double staining for NeuN (green)/LC3 (red) of sections from the spinal cord in each group (white arrows: LC3-positive neurons). (E) Quantification of the number of LC3-positive neurons in each group ( n = 5). (F) Double staining for NeuN (green)/p62 (red) of sections from the spinal cord in each group. (G) Quantification of the number of p62-positive neurons in each group ( n = 5). * P < 0.05 vs. SCI group, ** P < 0.01 vs. SCI group; && P < 0.01 vs. Netrin-1 group.

Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury

Journal: Scientific Reports    PubMed ID: 28186165    Data: 2017/2/10

Authors: Liangjie Bai, Xifan Mei, Gang Lv

Article Snippet:Recombinant rat Netrin-1 was purchased from Creative BioMart (Shirley, NY, USA).. Anti-LC3B, anti-Beclin-1, anti-NeuN, anti-mTOR, and anti-p-mTOR antibody as well as goat anti-rabbit and goat anti-mouse-IgG HRP were purchased from Abcam (Cambridge, MA, USA).Anti-LC3B, anti-Beclin-1, anti-NeuN, anti-mTOR, and anti-p-mTOR antibody as well as goat anti-rabbit and goat anti-mouse-IgG HRP were purchased from Abcam (Cambridge, MA, USA).

BBB scores of the sham, SCI, and Netrin-1 groups were evaluated at 0, 1, 3, 7, 14, and 21 days after contusion. *P < 0.05 vs. the SCI group; **P < 0.01 vs. the SCI group. Data represented mean ± SEM (n = 6).

(a) Expression of AMPK, p-AMPK, mTOR, p-mTOR, p-P70S6K, P70S6K, Beclin-1, LC3B, and β-tubulin in the sham, SCI, Netrin-1, Netrin-1+ compound C, and compound C groups. (b,c,d) Quantification analysis of p-mTOR/mTOR p-P70S6K/P70S6K, p-AMPK/AMPK, LC3B-II/LC3B-I, and Beclin-1/β-tubulin in each group. Mean ± SD, n = 3. *P < 0.05 vs. the SCI group; **P < 0.01 vs. the SCI group; & P < 0.05 vs. the Netrin-1+ compound C group; && P < 0.01 vs. the Netrin-1+ compound C group.

(a) Double staining for NeuN (green)/LC3B (red) of sections from the spinal cord sample in the sham, SCI, and Netrin-1 groups. Scale bar = 100 μm (white arrows: LC3B-positive neurons). (b) Quantification of the number of LC3B-positive neurons in each group. Data were represented as mean ± SD, n = 5. **P < 0.01 vs. the SCI group; ## P < 0.01 vs. the sham group.