Recombinant Rat Ntn1 protein, His/GST-tagged
Cat.No. : | Ntn1-257R |
Product Overview : | Recombinant Rat Ntn1(Pro313~Pro565) fused with two N-terminal Tags, His-tag and GST-tag was expressed in E. coli. |
Availability | July 02, 2025 |
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Species : | Rat |
Source : | E.coli |
Tag : | GST&His |
Protein Length : | Pro313~Pro565 |
Description : | Ntn1 played an important role in many functions. |
Form : | Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
Molecular Mass : | 60.5kDa |
AA Sequence : | PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSMEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIAXKCPKIK PLKKYLLLGN AEDSP |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method) |
Purity : | > 90% |
Applications : | SDS-PAGE; WB; ELISA; IP. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4. |
Publications : |
Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury (2017)
Neuroprotection of netrin-1 on neurological recovery via Wnt/β-catenin signaling pathway after spinal cord injury (2020)
The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats (2017)
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Gene Name | Ntn1 netrin 1 [ Rattus norvegicus ] |
Official Symbol | Ntn1 |
Synonyms | NTN1; netrin 1; netrin-1; |
Gene ID | 114523 |
mRNA Refseq | NM_053731 |
Protein Refseq | NP_446183 |
MIM | |
UniProt ID | |
Chromosome Location | 10q24 |
Pathway | Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; DCC mediated attractive signaling, organism-specific biosystem; |
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The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats
Journal: Frontiers in Cellular Neuroscience PubMed ID: 29209172 Data: 2017/11/3
Authors: Liangjie Bai, Xifan Mei, Gang Lv
Article Snippet:Recombinant rat Netrin-1 (Creative BioMart, Shirley, NY, USA) was dissolved in 1× phosphate-buffered saline (PBS) to achieve a final concentration of 800 ng/mL.. Compound C (Selleck Chemicals LLC, USA) was dissolved in 1× PBS and administered at a dose of 20 mg/kg (Bai et al., ).Compound C (Selleck Chemicals LLC, USA) was dissolved in 1× PBS and administered at a dose of 20 mg/kg (Bai et al., ).



Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury
Journal: Scientific Reports PubMed ID: 28186165 Data: 2017/2/10
Authors: Liangjie Bai, Xifan Mei, Gang Lv
Article Snippet:Recombinant rat Netrin-1 was purchased from Creative BioMart (Shirley, NY, USA).. Anti-LC3B, anti-Beclin-1, anti-NeuN, anti-mTOR, and anti-p-mTOR antibody as well as goat anti-rabbit and goat anti-mouse-IgG HRP were purchased from Abcam (Cambridge, MA, USA).Anti-LC3B, anti-Beclin-1, anti-NeuN, anti-mTOR, and anti-p-mTOR antibody as well as goat anti-rabbit and goat anti-mouse-IgG HRP were purchased from Abcam (Cambridge, MA, USA).

BBB scores of the sham, SCI, and

(a) Expression of AMPK, p-AMPK, mTOR, p-mTOR, p-P70S6K, P70S6K, Beclin-1, LC3B, and β-tubulin in the sham, SCI,

(a) Double staining for NeuN (green)/LC3B (red) of sections from the spinal cord sample in the sham, SCI, and
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