Active Recombinant Chicken NTN1 Protein, His-tagged

• Cat.No.: NTN1-66C
• Product Overview: Recombinant chicken NTN1(Gly26 - Ala606), with a C-terminal 10-His tag protein was expressed in mouse myeloma cell line.

Specification

• Source: Mammalian cells
• Species: Chicken
• Tag: His
• Predicted N Terminal: Gly26
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rcNetrin-1 with a linear range of 6-400 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 66.3 kDa
  SDS-PAGE: 75-85 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Reconstitution: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: NTN1
• Official Symbol: NTN1 netrin 1 [ Gallus gallus (chicken) ]
• Synonyms: NTN1; netrin 1; netrin-1; Netrin1; Netrin-1; NTN1Lnetrin 1, mouse, homolog of
• Gene ID: 396389
• mRNA Refseq: NM_205419
• Protein Refseq: NP_990750
• UniProt ID: Q90922