Recombinant Mouse Thioredoxin Reductase 1, His-tagged

• Cat.No.: Txnrd1-736M
• Product Overview: Recombinant mouse cytosolic thioredoxin with a 6-His tag is fully active with thioredoxin reductase. Mr is approximately 12,000 D.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3" UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
• Purity: Purified via immunoaffinity column chromatography, it contains no E. coli Thioredoxin-S2.
• Presentation: 100 μg of protein, salt-free, lyophilized from 0.5% NH4CO3.
• Reconstitution: Add 100 μL of filtered deionized water or physiological buffer to generate a 1 mg/mL solution.
• Storage: Store the lyophilized product at 2°- 8°C.

Gene Information

• Gene Name: Txnrd1 thioredoxin reductase 1 [ Mus musculus ]
• Synonyms: Txnrd1; thioredoxin reductase 1; TR; TR1; TrxR1; thioredoxin reductase 1, cytoplasmic; TR alpha; thioredoxin reductase TR1; EC 1.8.1.9
• Gene ID: 50493
• mRNA Refseq: NM_001042513
• Protein Refseq: NP_001035978
• UniProt ID: Q9JMH6
• Chromosome Location: 10 C1; 10
• Pathway: Pyrimidine metabolism
• Function: FAD binding; NADP or NADPH binding; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor; thioredoxin-disulfide reductase activity