Recombinant Rattus Clusterin, His-tagged, T7-tagged

• Cat.No.: Clu-108R
• Product Overview: The Rat Clusterin was constructed as a recombinant protein with N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). The Clusterin Rat His-Tagged Fusion Protein, produced inE.coli, is 26.5 kDa protein containing 215 amino acid residues of the APO-J Rat and 25 additional amino acid residues – His-Tag, T7-Tag.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate and the chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil -helices and three predicted amphipathic a-helices. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others.
• Amino acid sequence: MASMTGGQQM GRDPNSSSPF YFWMNGDRID SLLESDRQQS QVLDAMQDSF TRASGIIDTL FQDRFFTHEP QDIHHFSPMG FPHKRPHLLY PKSRLVRSLM PLSHYGPLSF HNMFQPFFDM IHQAQQAMDV QLHSPALQFPDVDFLKEGED DRTVCKEIRH NSTGCLKMKG QCEKCQEILS VDCSTNNPAQ ANLRQELNDS LQVAERLTQQ YNELLHSLQS KMLNTSSLLE QALEHHHHHH
• Specificity: The amino acid sequence of the Clusterin Rat recombinant is 100% homologous to the amino acid sequence 146-360 of the Clusterin Rat precursor.
• Purification Method: Ni-NTA chromatography.
• Purity: Greater than 95% (SDS PAGE analyzed).
• Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris pH 7.2.
• Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
• Applications: Western blotting
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.

Gene Information

• Gene Name: Clu clusterin [ Rattus norvegicus ]
• Synonyms: Clu; Clusterin; CLI; APOJ; SGP2; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B; CLU; TRPM2; KUB1; AAG4; MGC24903; Dimeric acidic glycoprotein; DAG protein; Testosterone repressed prostate message-2; Sulfated glycoprotein-2; Complement lysis inhibitor;SP-40; MGC24903; Apolipoprotein J
• Gene ID: 24854
• mRNA Refseq: NM_053021
• Protein Refseq: NP_444180
• UniProt ID: P05371
• Chromosome Location: 15p12
• Function: protein binding