Active Recombinant Rhesus EPHA4 Protein, His-tagged

• Cat.No.: EPHA4-1176R
• Product Overview: Recombinant rhesus EPHA4 (NP_001247799.1) (Met1-Thr547) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: His
• Predicted N Terminal: Val 20
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Rhesus EPHA4-His at 10 μg/ml (100 μl/well) can bind mouse EFNA5-Fc, The EC50 of mouse EFNA5-Fc is 28.2-65.8 ng/ml.
• Molecular Mass: The recombinant rhesus EPHA4 comprises 539 amino acids and has a calculated molecular mass of 59.8 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA4 EPH receptor A4 [ Macaca mulatta ]
• Official Symbol: EPHA4
• Synonyms: ephrin type-A receptor 4
• Gene ID: 704857
• mRNA Refseq: NM_001260870
• Protein Refseq: NP_001247799
• Chromosome Location: chromosome: 12
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem