MERTK

  • Official Full Name

    c-mer proto-oncogene tyrosine kinase

  • Overview

    In case of filovirus infection, seems to function as a cell entry factor.
  • Synonyms

    MERTK; c-mer proto-oncogene tyrosine kinase; Eyk; Mer; Nyk; nmf12; tyrosine-protein kinase Mer; Tyro 12; proto-oncogene c-Mer; receptor tyrosine kinase MerTK; proto-oncogene tyrosine-protein kinase Mer;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Cynomolgus
  • Dog
  • Human
  • Mouse
  • Rat
  • E.coli
  • HEK293
  • HEK293T
  • Human Cell
  • Insect cell
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Sf9 Insect Cell
  • Wheat Germ
  • C
  • His
  • DDK
  • DDK|His
  • Fc
  • Fc\Avi
  • Flag
  • GST
  • hFc
  • Avi
  • His (Fc)
  • His|T7
  • Myc
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MERTK-150H Active Recombinant Human MERTK protein, Fc-tagged Insect Cell Fc
Human MERTK-2180H Active Recombinant Human MERTK protein, Fc-tagged HEK293 Fc Ala 21 - Ile 505
Human MERTK-565H Recombinant Human MERTK Protein, DDK/His-tagged HEK293 DDK/His
Human MERTK-30094TH Recombinant Human MERTK N/A
Human MERTK-1183H Recombinant Human MERTK protein, His & T7-tagged E.coli His/T7 Leu587~Leu858 (Accession # Q12866)
Human MERTK-149H Recombinant Human MERTK protein, His-tagged HEK293 His Met177-Ala499
Human MERTK-566H Recombinant Human MERTK Protein, DDK-tagged Sf9 Insect Cell DDK
Human MERTK-148H Recombinant Human MERTK protein HEK293 N/A Met 1-Ala 499
Human MERTK-1352H Recombinant Human MERTK protein(Glu578-Tyr872), His&GST-tagged Insect Cells N-His&GST Glu578-Tyr872
Human MERTK-147H Recombinant Human MERTK protein, His&hFc-tagged HEK293 His&hFc Met1-Ala499
Human MERTK-472H Recombinant Human MERTK, Gly & Pro tagged Insect Cell His
Human MERTK-258H Recombinant Human MERTK, GST-tagged, Active Sf9 Insect Cell GST
Human MERTK-484HCL Recombinant Human MERTK cell lysate Insect Cell N/A
Human MERTK-001HCL Recombinant Human MERTK cell lysate Insect Cell N/A
Human MERTK-2888HCL Recombinant Human MERTK cell lysate Human Cell N/A
Human MERTK-03H Recombinant Human MERTK Protein, hFc-Avi tagged, Biotinylated Human Cell hFc-Avi Arg26-Ala499
Human MERTK-3902H Recombinant Human MERTK Protein (Arg21-Ala494), C-His tagged Mammalian cells C-His Arg21-Ala494
Human MERTK-1397H Recombinant Human MERTK Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human MERTK-900HFL Recombinant Full Length Human MERTK Protein, C-Flag-tagged Mammalian cells Flag
Human MERTK-1397H-B Recombinant Human MERTK Protein Pre-coupled Magnetic Beads HEK293
Human MERTK-3903H Recombinant Human MERTK Protein (Leu587-Leu858), N-His tagged E.coli N-His Leu587-Leu858
Human MERTK-3218H Recombinant Human MERTK protein(Glu578-Tyr872) Insect Cells N/A Glu578-Tyr872
Human MERTK-4437H Active Recombinant Human MERTK Protein, GST-tagged Insect Cell GST
Human MERTK-4436H Recombinant Human MERTK Protein, GST-tagged Wheat Germ GST
Human MERTK-950H Recombinant Human MERTK Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human MERTK-92H Recombinant Human MERTK protein, His-tagged HEK293 His Ala21-Ile505
Human MERTK-175H Recombinant Human MERTK Protein, Fc\Avi-tagged Human Cell Fc\Avi
Mouse Mertk-1149M Recombinant Mouse Mertk protein, hFc-tagged HEK293 hFc Met1-Phe498
Mouse Mertk-7397M Active Recombinant Mouse Mertk protein(Glu573-Tyr867), His&GST-tagged Insect Cells N-His&GST Glu573-Tyr867
Mouse MERTK-413MCL Recombinant Mouse MERTK cell lysate Insect Cell N/A
Mouse MERTK-1816MCL Recombinant Mouse MERTK cell lysate Human Cell N/A
Mouse Mertk-4035M Recombinant Mouse Mertk Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse MERTK-176M Recombinant Mouse MERTK Protein, Fc-tagged Human Cell Fc
Mouse MERTK-2194M Recombinant Mouse MERTK protein, Fc-tagged HEK293 Fc Gly 19 - Met 497
Mouse Mertk-04M Recombinant Mouse Mertk Protein, hFc tagged, Biotinylated Human Cell hFc Glu23 - Phe498
Mouse MERTK-0515M Recombinant Mouse MERTK protein(19-497aa), His-tagged HEK293 C-His 19-497aa
Rat MERTK-3650R Recombinant Rat MERTK Protein Mammalian Cell His
Rat MERTK-3306R Recombinant Rat MERTK Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat Mertk-5041R Recombinant Rat Mertk protein, His-tagged HEK293 His 19-497aa
Rat MERTK-3306R-B Recombinant Rat MERTK Protein Pre-coupled Magnetic Beads HEK293
Dog Mertk-001D Recombinant Dog MERTK protein, GST-tagged Insect cell GST
Cynomolgus MERTK-2195C Recombinant Cynomolgus MERTK protein, Fc-tagged HEK293 Fc Ala 23- Ile 507
Chicken MERTK-6546C Recombinant Chicken MERTK Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
MERTK-9.jpg

Fig1. An illustration of the MERTK gene showing the different exons coding for the different protein domains of the receptor. Exons are labeled 1 to 19. (Almedawar, 2020)

What is MERTK protein?

MERTK (MER proto-oncogene, tyrosine kinase) gene is a protein coding gene which situated on the long arm of chromosome 2 at locus 2q13. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. The MERTK protein is consisted of 999 amino acids and its molecular mass is approximately 110.2 kDa.

What is the function of MERTK protein?

Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

MERTK Related Signaling Pathway

MERTK proteins are involved in several signaling pathways, the most important of which are the MERK-PI3K-Akt and MERK-STAT3 signaling pathways. In the MERK-PI3K-Akt pathway, MERTK further activates protein kinase B (Akt) by activating phosphatidylinositol 3 kinase (PI3K), thereby promoting cell survival and proliferation. In the MERK-STAT3 pathway, MERTK regulates cell proliferation, aging and immune response by activating signal transduction and transcriptional activator 3 (STAT3).

MERTK Related Diseases

Diseases associated with MERTK dysfunction include hereditary retinal dystrophy, hemostatic disorders, and some types of leukemia. Second, MERTK is also closely associated with the development of tumors, including breast cancer, lung cancer, ovarian cancer and glioma. In addition, MERTK has been linked to autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. MERTK is also involved in the development of cardiovascular diseases, such as atherosclerosis, and neurological diseases, such as Parkinson's disease and cerebral ischemia.

Bioapplications of MERTK

In the aspect of tumor therapy, MERTK, as an important target, is being studied as the targeted therapy of new anticancer drugs. In addition, MERTK has important applications in the field of ophthalmology, especially in diseases related to macular degeneration, where MERTK is considered a potential therapeutic target.

MERTK-10.jpg

Fig2. Strategies for anti-Mertk therapeutics. A variety of strategies are possible for targeting Mertk therapeutically. (Kevin C Lahey, 2022)

Case study 1: Seba Almedawar, 2020

Maintenance of a healthy photoreceptor-retinal pigment epithelium (RPE) interface is essential for vision. At the center of this interface, apical membrane protrusions stemming from the RPE ensheath photoreceptor outer segments (POS), and are possibly involved in the recycling of POS through phagocytosis. The molecules that regulate POS ensheathment and its relationship to phagocytosis remain to be deciphered.

By means of ultrastructural analysis, the researchers revealed that MERTK ligands, GAS6 and PROS1, rather than αVβ5 integrin receptor ligands, triggered POS ensheathment by human embryonic stem cell (hESC)-derived RPE. Consistently, POS ensheathment, fragmentation, and internalization were abolished in MERTK mutant RPE, and rescue of MERTK expression in retinitis pigmentosa (RP38) patient RPE counteracted these defects. The results suggest that loss of ensheathment due to MERTK dysfunction might contribute to vision impairment in RP38 patients.

MERTK-3.jpg

Fig1. Immunoblot (western blot) analysis of MERTK using total protein extracts from wild-type and MERTK mutant RPE.

MERTK-4.jpg
Fig2. MERTK Mutations in Human RPE Abolish POS Ensheathment. SEM images of wild-type and MERTK mutant RPE treated with F-POS and 30% serum. (E) Wild-type hESC-RPE. (F) MERTK mutant MERTK-RPE. (G) MERTK mutant EX2-RPE. (H) MERTK mutant EX14-RPE.

Case study 2: Frank C Cackowski, 2017

Many prostate cancer (PCa) recurrences are thought to be due to reactivation of disseminated tumor cells (DTCs). The researchers previously found a role of the TAM family of receptor tyrosine kinases TYRO3, AXL, and MERTK in PCa dormancy regulation. However, the mechanism and contributions of the individual TAM receptors is largely unknown.

Knockdown of MERTK, but not AXL or TYRO3 by shRNA in PCa cells induced a decreased ratio of P-Erk1/2 to P-p38, increased expression of p27, NR2F1, SOX2, and NANOG, induced higher levels of histone H3K9me3 and H3K27me3, and induced a G1/G0 arrest, all of which are associated with dormancy. Also knockdown of MERTK in PCa cells increased metastasis free survival in an intra-cardiac injection mouse xenograft model. MERTK knockdown also failed to inhibit PCa growth in vitro and subcutaneous growth in vivo. Overall, this study shows that MERTK stimulates PCa dormancy escape through a MAP kinase dependent mechanism, also involving p27, pluripotency transcription factors, and histone methylation.

MERTK-5.jpg

Fig3. Effect of MERTK knockdown on growth of prostate cancer cells in culture and in subcutaneous tumors.

MERTK-6.jpg
Fig4. Control or shMER PC3 cells cultured with 5 μM SB203580 or 0.05% DMSO solvent control for 14 days in 10% serum and 3 days in 0.1% serum followed by cell cycle analysis with total DNA and BrdU labeling as described.
MERTK-7.jpg

Fig1. Updated pathogenetic model of IgG4-RD Apoptotic phenomena triggered by CD4+ CTLs activate scavenger MerTK+ macrophages through ProS1 ligation. (Lucrezia Rovati, 2021)

MERTK-8.jpg

Fig2. MERTK stimulates PCa dormancy escape through a MAP kinase dependent mechanism, also involving p27, pluripotency transcription factors, and histone methylation. (Frank C Cackowski, 2017)

MERTK involved in several pathways and played different roles in them. We selected most pathways MERTK participated on our site, such as Cell surface interactions at the vascular wall, Hemostasis, which may be useful for your reference. Also, other proteins which involved in the same pathway with MERTK were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Cell surface interactions at the vascular wallSLC16A8;CEACAM1;SLC7A6;SIRPA;SLC7A11;GRB7;AMICA1;F11R.2;GAS6
HemostasisITPK1;HBD;CLU;TRPC6A;LRRC16A;ANXA2;GATA1A;CFHL3;DGKB

MERTK has several biochemical functions, for example, ATP binding, protein binding, transmembrane receptor protein tyrosine kinase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by MERTK itself. We selected most functions MERTK had, and list some proteins which have the same functions with MERTK. You can find most of the proteins on our site.

Function Related Protein
ATP bindingERN1;ATP2B3A;SUPV3L1;CKBA;KIF3B;ADCY1;MAT2AL;NEK3;UBE2D4
protein bindingIMPA1;GLS2;FAM32A;NMNAT1;SF3A2;IRS2;HHLA2;LRRC20;WIPF2
transmembrane receptor protein tyrosine kinase activityEPHA4B;KDRL;ERBB4A;CADM2B;ALK;ERBB4;CSF1RA;FLT3;RYK

MERTK has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MERTK here. Most of them are supplied by our site. Hope this information will be useful for your research of MERTK.

saicar; PKM; abl-tide; HSP90AB1; q5nhh0_fratt; mpl; crizotinib

Research Area

Related articles

Albert, R; Kristof, E; et al. Triamcinolone regulated apopto-phagocytic gene expression patterns in the clearance of dying retinal pigment epithelial cells. A key role of Mertk in the enhanced phagocytosis. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1850:435-446(2015).
Ginisty, A; Gely-Pernot, A; et al. Evidence for a Subventricular Zone Neural Stem Cell Phagocytic Activity Stimulated by the Vitamin K-Dependent Factor Protein S. STEM CELLS 33:515-525(2015).
  • Q&As
  • Reviews

Q&As (5)

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In cancer biology, how does MERTK contribute to tumor progression and metastasis, and are there potential therapeutic strategies targeting MERTK in cancer treatment? 02/18/2022

MERTK's involvement in cancer includes promoting tumor cell survival and metastasis. Targeting MERTK is under investigation as a potential therapeutic strategy in cancer treatment.

How does the Gas6-MERTK signaling axis contribute to cellular processes beyond phagocytosis, and what downstream effectors are activated upon MERTK activation? 07/27/2021

The Gas6-MERTK signaling axis influences diverse cellular processes, activating downstream effectors such as PI3K/Akt and MAPK pathways. Understanding these signaling events provides insights into MERTK's functional diversity.

In neurobiology, what is the role of MERTK in microglial phagocytosis and synaptic pruning, and how does this impact neural circuitry development? 03/10/2020

MERTK is involved in microglial phagocytosis and synaptic pruning, contributing to neural circuitry development. Dysregulation may affect synaptic connectivity, with implications for neurodevelopmental disorders.

What is the role of MERTK in the regulation of immune responses, and how does its dysregulation contribute to autoimmune disorders and inflammatory diseases? 09/03/2019

MERTK is involved in immune regulation, influencing inflammatory responses. Dysregulation of MERTK has been implicated in autoimmune disorders, emphasizing its role in maintaining immune homeostasis.

What experimental techniques are commonly used to study MERTK, including its ligands, downstream signaling pathways, and cellular functions? 12/04/2018

Techniques such as co-immunoprecipitation, flow cytometry, and advanced imaging methods are commonly used to study MERTK, providing insights into ligand interactions, signaling pathways, and cellular functions.

Customer Reviews (3)

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04/08/2022

    Fast shipping and excellent service. This product is a lab essential.

    06/15/2020

      Highly recommend for quality and prompt delivery.

      03/25/2018

        Exceptional product, outstanding customer service!

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