Recombinant Human MERTK cell lysate

Cat.No. : MERTK-2888HCL
Product Overview : Human Mer / MERTK derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : MERTK c-mer proto-oncogene tyrosine kinase [ Homo sapiens ]
Official Symbol : MERTK
Synonyms : MERTK; c-mer proto-oncogene tyrosine kinase; tyrosine-protein kinase Mer; mer; RP38; STK kinase; proto-oncogene c-Mer; MER receptor tyrosine kinase; receptor tyrosine kinase MerTK; MER; c-mer; MGC133349;
Gene ID : 10461
mRNA Refseq : NM_006343
Protein Refseq : NP_006334
MIM : 604705
UniProt ID : Q12866
Chromosome Location : 2q14.1
Pathway : Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem;
Function : ATP binding; nucleotide binding; receptor activity; transmembrane receptor protein tyrosine kinase activity;

Not For Human Consumption!

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04/08/2022

    Fast shipping and excellent service. This product is a lab essential.

    06/15/2020

      Highly recommend for quality and prompt delivery.

      03/25/2018

        Exceptional product, outstanding customer service!

        Q&As (5)

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        In cancer biology, how does MERTK contribute to tumor progression and metastasis, and are there potential therapeutic strategies targeting MERTK in cancer treatment? 02/18/2022

        MERTK's involvement in cancer includes promoting tumor cell survival and metastasis. Targeting MERTK is under investigation as a potential therapeutic strategy in cancer treatment.

        How does the Gas6-MERTK signaling axis contribute to cellular processes beyond phagocytosis, and what downstream effectors are activated upon MERTK activation? 07/27/2021

        The Gas6-MERTK signaling axis influences diverse cellular processes, activating downstream effectors such as PI3K/Akt and MAPK pathways. Understanding these signaling events provides insights into MERTK's functional diversity.

        In neurobiology, what is the role of MERTK in microglial phagocytosis and synaptic pruning, and how does this impact neural circuitry development? 03/10/2020

        MERTK is involved in microglial phagocytosis and synaptic pruning, contributing to neural circuitry development. Dysregulation may affect synaptic connectivity, with implications for neurodevelopmental disorders.

        What is the role of MERTK in the regulation of immune responses, and how does its dysregulation contribute to autoimmune disorders and inflammatory diseases? 09/03/2019

        MERTK is involved in immune regulation, influencing inflammatory responses. Dysregulation of MERTK has been implicated in autoimmune disorders, emphasizing its role in maintaining immune homeostasis.

        What experimental techniques are commonly used to study MERTK, including its ligands, downstream signaling pathways, and cellular functions? 12/04/2018

        Techniques such as co-immunoprecipitation, flow cytometry, and advanced imaging methods are commonly used to study MERTK, providing insights into ligand interactions, signaling pathways, and cellular functions.

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