Recombinant Mouse MERTK protein(19-497aa), His-tagged

• Cat.No.: MERTK-0515M
• Product Overview: Recombinant Mouse MERTK protein(Q60805)(19-497aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: Gly19-Met497
• Form: Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Mertk at 2 μg/mL can bind anti-MERTK recombinant antibody, the EC50 is 19.22-25.80 ng/mL.
• Molecular Mass: 55.0 kDa
• AASequence: GGTAEKWEETELDQLFSGPLPGRLP VNHRPFSAPHSSRDQLPPPQTGRSH PAHTAAPQVTSTASKLLPPVAFNHT IGHIVLSEHKNVKFNCSINIPNTYQ ETAGISWWKDGKELLGAHHSITQFY PDEEGVSIIALFSIASVQRSDNGSY FCKMKVNNREIVSDPIYVEVQGLPY FIKQPESVNVTRNTAFNLTCQAVGP PEPVNIFWVQNSSRVNEKPERSPSV LTVPGLTETAVFSCEAHNDKGLTVS KGVHINIKVIPSPPTEVHILNSTAH SILVSWVPGFDGYSPLQNCSIQVKE ADRLSNGSVMVFNTSASPHLYEIQQ LQALANYSIAVSCRNEIGWSAVSPW ILASTTEGAPSVAPLNITVFLNESN NILDIRWTKPPIKRQDGELVGYRIS HVWESAGTYKELSEEVSQNGSWAQI PVQIHNATCTVRIAAITKGGIGPFS EPVNIIIPEHSKVDYAPSSTPAPGN TDSM
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Gene Information

• Gene Name: Mertk c-mer proto-oncogene tyrosine kinase [ Mus musculus ]
• Official Symbol: MERTK
• Synonyms: MERTK; c-mer proto-oncogene tyrosine kinase; tyrosine-protein kinase Mer; Tyro 12; proto-oncogene c-Mer; receptor tyrosine kinase MerTK; proto-oncogene tyrosine-protein kinase Mer; Eyk; Mer; Nyk; nmf12
• Gene ID: 17289
• mRNA Refseq: NM_008587
• Protein Refseq: NP_032613

Reviews

04/08/2022

Fast shipping and excellent service. This product is a lab essential.

06/15/2020

Highly recommend for quality and prompt delivery.

03/25/2018

Exceptional product, outstanding customer service!

Q&As

In cancer biology, how does MERTK contribute to tumor progression and metastasis, and are there potential therapeutic strategies targeting MERTK in cancer treatment?

MERTK's involvement in cancer includes promoting tumor cell survival and metastasis. Targeting MERTK is under investigation as a potential therapeutic strategy in cancer treatment.

How does the Gas6-MERTK signaling axis contribute to cellular processes beyond phagocytosis, and what downstream effectors are activated upon MERTK activation?

The Gas6-MERTK signaling axis influences diverse cellular processes, activating downstream effectors such as PI3K/Akt and MAPK pathways. Understanding these signaling events provides insights into MERTK's functional diversity.

In neurobiology, what is the role of MERTK in microglial phagocytosis and synaptic pruning, and how does this impact neural circuitry development?

MERTK is involved in microglial phagocytosis and synaptic pruning, contributing to neural circuitry development. Dysregulation may affect synaptic connectivity, with implications for neurodevelopmental disorders.

What is the role of MERTK in the regulation of immune responses, and how does its dysregulation contribute to autoimmune disorders and inflammatory diseases?

MERTK is involved in immune regulation, influencing inflammatory responses. Dysregulation of MERTK has been implicated in autoimmune disorders, emphasizing its role in maintaining immune homeostasis.

What experimental techniques are commonly used to study MERTK, including its ligands, downstream signaling pathways, and cellular functions?

Techniques such as co-immunoprecipitation, flow cytometry, and advanced imaging methods are commonly used to study MERTK, providing insights into ligand interactions, signaling pathways, and cellular functions.