Recombinant Human MERTK cell lysate
Cat.No. : | MERTK-484HCL |
Product Overview : | Human Mer / MERTK derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | MERTK c-mer proto-oncogene tyrosine kinase [ Homo sapiens ] |
Official Symbol : | MERTK |
Synonyms : | MERTK; c-mer proto-oncogene tyrosine kinase; tyrosine-protein kinase Mer; mer; RP38; STK kinase; proto-oncogene c-Mer; MER receptor tyrosine kinase; receptor tyrosine kinase MerTK; MER; c-mer; MGC133349; |
Gene ID : | 10461 |
mRNA Refseq : | NM_006343 |
Protein Refseq : | NP_006334 |
MIM : | 604705 |
UniProt ID : | Q12866 |
Chromosome Location : | 2q14.1 |
Pathway : | Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; |
Function : | ATP binding; nucleotide binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; |
Products Types
◆ Recombinant Protein | ||
MERTK-3218H | Recombinant Human MERTK protein(Glu578-Tyr872) | +Inquiry |
MERTK-950H | Recombinant Human MERTK Protein, MYC/DDK-tagged | +Inquiry |
MERTK-1352H | Recombinant Human MERTK protein(Glu578-Tyr872), His&GST-tagged | +Inquiry |
MERTK-03H | Recombinant Human MERTK Protein, hFc-Avi tagged, Biotinylated | +Inquiry |
MERTK-1397H | Recombinant Human MERTK Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
MERTK-001HCL | Recombinant Human MERTK cell lysate | +Inquiry |
MERTK-413MCL | Recombinant Mouse MERTK cell lysate | +Inquiry |
MERTK-1816MCL | Recombinant Mouse MERTK cell lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewFast shipping and excellent service. This product is a lab essential.
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Q&As (5)
Ask a questionMERTK's involvement in cancer includes promoting tumor cell survival and metastasis. Targeting MERTK is under investigation as a potential therapeutic strategy in cancer treatment.
The Gas6-MERTK signaling axis influences diverse cellular processes, activating downstream effectors such as PI3K/Akt and MAPK pathways. Understanding these signaling events provides insights into MERTK's functional diversity.
MERTK is involved in microglial phagocytosis and synaptic pruning, contributing to neural circuitry development. Dysregulation may affect synaptic connectivity, with implications for neurodevelopmental disorders.
MERTK is involved in immune regulation, influencing inflammatory responses. Dysregulation of MERTK has been implicated in autoimmune disorders, emphasizing its role in maintaining immune homeostasis.
Techniques such as co-immunoprecipitation, flow cytometry, and advanced imaging methods are commonly used to study MERTK, providing insights into ligand interactions, signaling pathways, and cellular functions.
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