Acitve Recombinant Human IL1R2 protein(Met1-Glu343), hFc-tagged

• Cat.No.: IL1R2-1263H
• Product Overview: Recombinant Human IL1RII(NP_004624.1)(Met 1-Glu 343) was expressed in HEK293 with C-terminal fused Fc region of human IgG1.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Glu343
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL1B at 2 μg/ml (100ul/well) can bind recombinant Human IL1R2-Fc with a linear range of 6.25-400 ng/ml. 2. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) bound IL-1 beta Protein, Human, Recombinant was 6.0 nM. 3. Measured by its ability to inhibit Interferon gamma secretion by Human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The EC50 for this effect is typically 0.1-0.8 μg/mL.
• Molecular Mass: The recombinant Human IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 568 amino acids and has a predicted molecular mass of 64.5 kDa. As a result of glycosylation, the rh IL1R2/Fc monomer migRates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL1R2 interleukin 1 receptor, type II [ Homo sapiens ]
• Official Symbol: IL1R2
• Synonyms: IL1R2; interleukin 1 receptor, type II; IL1RB; interleukin-1 receptor type 2; CD121b; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2; IL-1R-beta; antigen CDw121b; IL-1 type II receptor; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; type II interleukin-1 receptor, beta; MGC47725
• Gene ID: 7850
• mRNA Refseq: NM_004633
• Protein Refseq: NP_004624
• MIM: 147811
• UniProt ID: P27930