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Recombinant Mouse IL1R2 cell lysate

Cat.No. : IL1R2-1376MCL
Product Overview : Mouse IL1R2 / CD121b derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il1r2 interleukin 1 receptor, type II [ Mus musculus ]
Official Symbol : IL1R2
Synonyms : IL1R2; interleukin 1 receptor, type II; interleukin-1 receptor type 2; IL-1R-2; IL-1RT2; IL-1RT-2; IL-1R-beta; IL-1 type II receptor; IL-1 receptor beta chain; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; CD121b; Il1r-2;
Gene ID : 16178
mRNA Refseq : NM_010555
Protein Refseq : NP_034685
Pathway : Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem;
Function : interleukin-1 receptor activity; interleukin-1, Type II, blocking receptor activity; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What are the functional implications of IL1R2's intracellular domain, and how can engineered receptor constructs shed light on its contributions? 07/14/2021

The intracellular domain of IL1R2 has functional significance; engineered constructs clarify its role.

How does IL1R2's glycosylation affect its interaction with IL-1 ligands, and how can glycoproteomics methods elucidate these glycosylation sites? 04/29/2021

Glycosylation of IL1R2 affects its IL-1 ligand interaction, and glycoproteomics methods can reveal critical glycosylation sites.

In what ways can engineered IL1R2 variants with altered domains provide insights into its molecular mechanisms and potential therapeutic applications? 01/29/2021

Engineered IL1R2 variants offer insights into its mechanisms and therapeutic potential.

In what ways does IL1R2's presence influence the downstream signaling of IL-1 ligands through its competition with IL1R1, and how can genetic manipulation techniques help unravel this interplay? 01/04/2020

IL1R2's presence impacts IL-1 ligand signaling through competition with IL1R1; genetic manipulations unveil this interaction.

How do post-translational modifications, particularly ubiquitination, influence IL1R2's stability and turnover? 05/28/2019

Post-translational modifications, including ubiquitination, influence IL1R2's stability.

How does IL1R2's expression respond to inflammatory cues, and what role do transcription factors play in its regulatory network? 03/14/2019

IL1R2 expression responds to inflammation; transcription factors play a role in its regulation.

What insights can super-resolution microscopy offer regarding IL1R2's intracellular trafficking and subcellular localization dynamics? 01/06/2019

Super-resolution microscopy helps understand IL1R2's trafficking and subcellular dynamics in detail.

What specific structural features of IL1R2 enable it to function as a decoy receptor, and how can X-ray crystallography provide a detailed understanding of its ligand binding mechanisms? 10/09/2018

Specific structural elements of IL1R2 contribute to its decoy role; X-ray crystallography provides insights into ligand binding.

How does the shedding of IL1R2's ectodomain contribute to its role as a soluble decoy receptor, and which enzymes are involved in this process? 01/28/2018

Ectodomain shedding contributes to IL1R2's soluble decoy role; specific enzymes are involved.

What significance does IL1R2's interaction with IL-1 receptor accessory proteins hold in terms of its decoy function? 06/05/2016

IL1R2's interaction with accessory proteins is significant for its decoy function.

Customer Reviews (3)

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Reviews
10/10/2022

    High specificity.

    09/12/2020

      Good for flow cytometry.

      09/20/2016

        Suitable for co-immunoprecipitation.

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